1180 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



ally, also Cicer arietinum L.; Desmodium 

 canadense (L.) DC; Lathyrus sativus L., 

 grass pea; Litpiwws alhus L., white lupine ; 

 L. angiistijolius , blue lupine; L. densi- 

 floriis Benth. ; L. hartwegii Lindl. ; L. na- 

 nus Dougl.; Medicago arahica Huds., 

 spotted bur clover; M. hispida Gaertn., 

 toothed bur clover; Melilotus alba Desr., 

 white sweet clover; M. indicaAU., annual 

 yellow sweet clover; M. officinalis (L.) 

 Lam., yellow sweet clover; Phaseolus 

 acutijolius Gray, tepary bean; P. vulgaris 

 L., bean; Trifolium agrarium L. ; T. caro- 

 linianum Michx.; T. duhi^im Sibth.; 7'. 

 glomeratum L., cluster clover; T. hy- 

 bridurn L., alsike clover; T. incarnatinn 

 L., crimson clover; T. procumbcns L. ; T. 

 reflexum L.; T. suaveolens, Persian 

 clover ; Vicia saliva L., common vetch. 



Insusceptible species: All tested spe- 

 cies in families other than the Leguni- 

 inosae. 



Geographical distribution: United 

 States, British Isles, Europe, New 

 Zealand . 



Induced disease : In pea, clearing of 

 veins in young leaves, followed by chloro- 

 sis of newly formed leaves, stunting of 

 plant, and systemic chlorotic mottling. 

 In sweet pea, systemic chlorosis and 

 chlorotic mottling, flower colors broken. 

 In lupine, necrotic streak on one side of 

 stem, stunting of plant and bending of 

 growing point to injured side. Plant 

 soon wilts and dies. In Vicia faba, mot- 

 tled leaves contain characteristic iso- 

 metric crj^stals in host -cell nuclei (espe- 

 cially within nucleoli) as well as in cell 

 cytoplasm. • 



Transmission : By inoculation of ex- 

 pressed juice, with ease. Bj' aphids, 

 Macrosiphian pisi Kalt., M. solanifolii 

 Ashm. (= M. gci Koch), and Aphis 

 rumicis Linn. (APHIDIDAE). Not 

 transmitted through seed. 



Serological relationships : Specific pre- 

 cipitin reactions differentiate this virus 

 from tobacco -mosaic virus, tobacco-etch 

 virus, potato-mottle virus, potato mild- 

 mosaic virus, potato aucuba-mosaic virus, 

 and tobacco -ringspot virus. 



Thermal inactivation : At 60° C in 10 

 minutes. 



Literature: Chester, Phytopath., 25, 



1935, 68G-701 ; Doolittle and Jones, ibid., 

 15, 1925, 763-772 ; Johnson and Jones, Jour. 

 Agr. Res., 54, 1937, 629-638; McWhorter, 

 Phytopath., 31, 1941, 760-761; Murphy 

 and Pierce, ibid., 27, 1937, 710-721; Os- 

 born, ibid., 27, 1937, 589-603; Pierce, 

 Jour. Agr. Res., 51, 1935, 1017-1039; 

 Spierenburg, Tijdschr. Plantenz., 4^, 



1936, 71-76; Zaumeyer and Wade, Jour. 

 Agr. Res., 53, 1936, 161-185. 



20. Marmor pisi H. {loc. cit., 90). 

 From Ivatin pisum, pea. 



Common name : Pea enation-mosaic 

 virus. 



Hosts: LEGUMINOSAE— Pisum sa- 

 tivum L., pea; Vicia faba L., broad bean. 

 Experimentally, also Lathyrus odoratus 

 L., sweet pea; Soja max (L.) Piper, soy 

 bean; Trifolium incarnatum L., crimson 

 clover. 



Insusceptible species: LEGUMINO- 

 SAE — Arachis hypogaea L., peanut; 

 Medicago sativa L., alfalfa; Melilotus alba 

 Desr., white sweet clover; M. officinalis 

 (L.) Lam., yellow sweet clover; Phaseo- 

 lus aureus Roxb., mung bean; P. vulgaris 

 L., bean; Trifolium hybridum L., alsike 

 clover; T. pratense L., red clover; T. 

 repens L., white Dutch clover. SOL- 

 A NACEAE — Lycopersicon esculentum 

 Mill., tomato; Solanum tuberosum L., 

 potato . 



Geographical distribution: United 

 States, perhaps Germany. 



Induced disease: In peas, systemic 

 chlorotic mottling; in some varieties, as 

 Alderman, occasional necrotic spots and 

 numerous enations on lower surfaces of 

 leaves. Pods distorted. In broad bean, 

 systemic chlorotic spotting and striping 

 of leaves. In sweet pea and soy bean, 

 experimentally, systemic chlorotic mot- 

 tling. 



Transmission : By inoculation of ex- 

 pressed juice, using carborundum; more 

 readily from aphid-inoculated plants than 

 from mechanically -inoculated plants. 



