1190 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



may utilize many other varieties of this 

 species as host. 



Common name: Alsike-clover mosaic 

 virus. 



Hosts : LEGUM I NOSAE—Trifoliiwi 

 hybridum L., alsike clover; Pisum sati- 

 vum L., pea (except the varieties Horal, 

 Perfection, and Surprise). Experimen- 

 tally, also Crotalaria striata DC; C. 

 retusa L.; and C. spectabilis Roth (the 

 two last-named species are reported to be 

 insusceptible to the type strain of the 

 virus, but susceptible to one or more of 

 the other tested strains) ; Lupintis albus 

 L. ; L. angvstifolius L.; Medicago saliva 

 L.; Melilotus alba Desr.; Phaseolus vul- 

 garis L., bean; Trifolium incarnatum L.; 

 T. pratense L.; Vicia faba L. 



Insusceptible species: SOLAN A- 

 CEAE — Datura stramonium L. ; Nico- 

 tiana glauca Graham; N. glutinosa L.; 

 A^. tabacujn L. ; Petiinia hybrida Vilm. 

 LEG UM I NOSAE— Phaseolus aureus 

 Roxb., mung bean; P. lunatus L., sieva 

 bean; Soja max (L.) Piper, soybean; 

 Trijoliuvi repens L., white clover; Vicia 

 saliva L., spring vetch. 



Induced disease : In pea and bean, ex- 

 perimentally, systemic chlorotic mot- 

 tling; some isolates kill inoculated leaves 

 and even cause death of infected plants. 



Transmission : By inoculation with ex- 

 pressed juice, at dilutions to 1:6000 or 

 1 : 8000. No insect vector is known. 



Thermal inactivation : At 60 to 65° C in 

 10 minutes; one strain at lower tempera- 

 ture, 54 to 58° C. 



Strains : Several strains have been dis- 

 tinguished by the severity of their effects 

 on host plants. These may be charac- 

 terized as follows: var. fastidiens, var. 

 nov., type variety, the first of the strains 

 to be described (originally known as 

 alsike clover mosaic virus 1 ), induces mild 

 disease in pea, does not infect red clover ; 

 var. viite, var. nov., described as pea 

 mosaic virus 4, induces mild symptoms 

 on pea, infects red clover; var. reprimens , 

 var. nov., described as pea mosaic virus 

 5, stunts peas severely; var. denudans, 

 var. nov., described as alsike clover mosaic 



virus 2, defoliates pea plants. Varietal 

 names from New hat'in fastidiens , epithet 

 of the species, and from Latin milis, mild ; 

 reprimere, to restrain; and denudare, to 

 denude ; all three in reference to induced 

 symptoms. 



Literature: Wade and Zaumeyer, Phy- 

 topath., 28, 1938, 505-511; Zaumeyer, 

 Jour. Agr. Res., 60, 1940, 433-452. 



38. Marmor iners spec. nov. From 

 Latin iners, sluggish or inert, in reference 

 to failure of the virus to spread systemi- 

 cally in certain of its hosts. 



('ommon name : Pea-streak virus. 



Hosts: LEGUM I NOSAE— Pisum sa- 

 tivum L., pea. Experimentally, also 

 Galega officinalis L., goat's rue; Glycine 

 soja Sieb. and Zucc, soyabean; Lathyrus 

 odoratus L., sweet pea; Lotus hispidus 

 Desf.; Lupinus angustifolius L., blue 

 lupin; L. lutevs L., yellow lupin; L. 

 mutabilis Sweet; Phaseolus vulgaris L., 

 bean; Trifolium arvense L., haresfoot 

 trefoil ; T. cernuum Brot . , nodding clover ; 

 T. fragiferum L., strawberry clover; T. 

 glomeratum L., cluster clover; T. hybri- 

 dum L., alsike clover; T. pratense L., red 

 clover; T. repens L., white clover; Vicia 

 villosa Roth., hairy vetch. CUCUR- 

 BITACEAE — Cucumis melo L., rock 

 melon; C. sativus L., cucumber; Cucur- 

 bita pepo L., marrow. 



Insusceptible species : CHENOPODI- 

 ACEAE — Spinacia oleracea L., spinach; 

 Beta vulgaris L., beet. COMPOS I TAE 

 — Calendula officinalis L., calendula; 

 Lactuca saliva L., lettuce ; Zinnia elegans 

 Jacq., zinnia. CRUCIFERAE—Brass- 

 ica napus L., swede; B. oleracea L., cab- 

 bage; B. rapa L., turnip; Matthiola in- 

 cana R. Br., stock; Raphayms sativus L., 

 radish; Sisymbrium officinale (L.) Scop., 

 hedge mustard. LEGUM I NOSAE— 

 Arachis hypogaea L., peanut; Lathyrus 

 latif alius L., perennial sweet pea; L. 

 pubescens Hook, and Arn., Argentine 

 sweet pea ; Lotus corniculatus L. ; Lupinus 

 arboreus Sims, tree lupin; Medicago 

 arabica Huds. ; M. sativa L., lucerne (al- 

 falfa) ; Phaseolus multiflorus Willd., run- 



