1192 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



lanceolata L. ; P. major L. POLYGON - 

 ACEAE — Rumex acetosella L. SOLA X- 

 ACEAE — Datura stramonium L. ; Lyco- 

 persicon esculentum Mill. ; Nicotiana 

 glutinosa L. ; N. rustica L. ; N. sylvestris 

 Spegaz. and Comes; N. tabacum L. ; 

 Solanum nigrum L. 



Geographical distribution : United 

 States (Washington). 



Induced disease : Experimentally, in 

 pea, developing leaves late in opening; 

 clearing of veins, chlorotic spotting, 

 stunting, chlorotic mottling; stipules 

 mottled; stems, pods, and seeds appear 

 normal. If pea-wilt virus {Marmor re- 

 pens Johnson) is also present, a severe 

 streak disease occurs. Intracellular in- 

 clusions absent. In bean, light yellow 

 spots and clearing of veins. In spinach, 

 severe chlorotic mottling, dwarfing. In 

 alfalfa, streaks of yellowing along veins, 

 chlorotic mottling. 



Transmission : By inoculation of ex- 

 pressed juice. By dodder, Cuscuta cam- 

 pestrisYunck. {CON VOLVU L ACEAE). 

 Not by pea aphid, Macrosiphum pisi 

 Kalt. (APHIDIDAE). No insect vec- 

 tor is known. 



Thermal inactivation : At 60 to 62° C 

 in 10 minutes. 



Filterability : Passes Berkefeld W filter 

 candle. 



Other properties : Infectious in dilu- 

 tion of 1:10,000 and after storage in ex- 

 pressed juice or dried tissues for one 

 month at about 25° C. 



Literature: Johnson, Phytopatli., 32, 

 1942, 103-116; Johnson and Jones, Jour. 

 Agr. Res., 54, 1937, 629-638; Pierce, ibid., 

 51, 1935, 1017-1039 ; Zaumeyer and Wade, 

 ibid., 51, 1935, 715-749. 



40. Marmor tritici H. {lac. cit., 61). 

 From Latin triticum, wheat. 



Common names : Wheat -mosaic virus, 

 wheat-rosette virus. 



Hosts: GRAM I NEAE— Triticum aes- 

 tivum L., wheat; Secale cereale L., rye. 

 Experimentally, also all tested species of 

 the tribe Hordeae ; Triticum compactum 

 Host ; T. turgidum L. ; T. durum Desf . ; 



T. dicoccum Schrank; T. spelta L. ; T. 

 polonicum L. ; T. monococcum L., Hor- 

 deum vulgar e L., barley. 



Insusceptible species : GRAMINEAE 

 — Bromus inermis Leyss., awnless brome- 

 grass (of the tribe Festuceae). 



Geographical distribution : United 

 States, Japan. 



Induced disease: In wheat, systemic 

 chlorotic mottling, with dwarfing in some 

 varieties; vacuolate, rounded intracellu- 

 lar bodies in diseased cells, usually close 

 to nucleus. Some selections of Harvest 

 Queen wheat are resistant. 



Transmission : Through soil ; remains 

 infectious in soil 6 or more years. By in- 

 oculation of expres.ied juice (needle punc- 

 tures in stem). Not through seeds or 

 stubble of diseased plants. No insect 

 vector is known. 



Thermal inactivation : Contaminated 

 soil becomes incapable of infecting wheat 

 plants if heated for 10 minutes at 60° C 

 though not if heated for the same length 

 of time at 50° C. 



Literature : Johnson, Science, 95, 1942, 

 610; McKinney, Jour. Agr. Res., 23, 1923, 

 771-800; U. S. Dept. Agr., Bull. 1361, 

 1925; U. S. Dept. Agr., Circ. 442, 1937; 

 Jour. Agr. Res., 40, 1930, 547-556; Mc- 

 Kinney et al., ibid., 26, 1923, 605-608; 

 Wada and Hukano, Agr. and Hort., 9, 



1934, 1778-1790 (Rev. Appl.Mycol., i.^, 



1935, 618, Abst.); Jour. Imp. Agr. Exp. 

 Sta., 3, 1937, 93-128 (Rev. Appl. Mycol., 

 16, 1937, 665, Abst.); Webb, Jour. Agr. 

 Res., 35, 1927, 587-614; 5ff, 1928, 53-75. 



41. Marmor graminis McKinney. 

 (Jour. Washington Acad. Sci., 34, 1944, 

 325.) From Latin gramen, grass. 



Common name : Bro me -grass mosaic 

 virus. 



Hosts: GRAMINEAE— Bromus iner- 

 mis Leyss., awnless brome-grass. Ex- 

 perimentally, also Triticum aestivum L., 

 wheat ; Avena sativa L., oat. 



Geographical distribution : United 

 States (Kansas). 



Induced disease : In awnless brome- 

 grass, systemic chlorotic mottling of the 



