1226 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



1. Borrelina bombycis Paillot. 

 (Compt. rend. Acad. Sci., Paris, 182, 

 1926, 182.) Frona Latin bombyx, silk- 

 worm. (Note : Coccus-like bodies sur- 

 rounded by non-staining substances, as- 

 sociated with the induced disease, re- 

 ceived the provisional name Chlamy- 

 dozoon bombycis from Prowazek, Arch. f. 

 Protistenkunde, 10, 1907, 363.) 



Common names : Silkworm-jaundice 

 virus, silkworm-grasserie virus, silkworm 

 \nlt virus, Gelbsucht virus, Fettsucht 

 \irus. 



Host: BOMB YCI DAE— Bombyx mori 

 (L.), silkworm. 



Geographical distribution: Japan, 

 Italy, France. 



Induced disease : In silkworm, after 

 prodromal period of 5 days or more, yel- 

 low spots on skin, polyhedral bodies in 

 blood, inactivity, loss of appetite, ir- 

 ritability, weakening of body facilitating 

 rupture from mechanical stress, eventual 

 death. 



Transmission : By feeding. Experi - 

 mentally, also by injection. 



Serological relationships : Specific ag- 

 glutination, precipitation, and comple- 

 ment fixation. 



Thermal inactivation : At 60° C in 15 

 to 20 minutes in blood. 



Filterability : Passes Berkefeld N and 

 V, Chamberland Li, Lo, and L3 filters. 



Other properties : May survive at least 

 2 years in dry state. Stable between 

 pH 5 and about pH 9. Sedimentation 

 constant 17 S. 



Literature: Aoki and Chigasaki, Cent, 

 f. Bakt., I Abt., Orig., 86, 1921, 481-485; 

 Glaser and Lacaillade, Am. Jour. Hyg., 

 20, 1934, 454-464; Glaser and Stanley, 

 Jour. Exp. Med., 77, 1943, 451-466; v. 

 Prowazek, Cent. f. Bakt., I Abt., Orig., 

 67, 1912, 268-284; Suzuki, Bull. Imperial 

 Kyoto Sericultural College, 1, 1929, 45- 

 75; Trager, Jour. Exp. Med., 61, 1935, 

 501-513. 



2. Borrelina eflGiciens spec. nov. From 



Latin efficiens, effective, in reference to 

 effectiveness of this virus in controlling 

 nun-moth infestations. 



Common names : Nun-moth disease 

 virus, nun-moth wilt virus, Wipfelkrank- 

 heit virus. 



Host: LYMANTRIIDAE—Lyman- 

 tria monacha (L.), nun moth. 



Geographical distribution: Europe. 



Induced disease : In eggs, larvae, pupae 

 and occasionally adults of nun moth, 

 polyhedral bodies in affected tissues. 

 Blood of sick larvae turbid; later, blood 

 cells few ; contents of body finally become 

 a gray-brown, semifluid mass. 



Transmission : By feeding. 



Thermal inactivation : At 55 to 60° C in 

 5 to 10 minutes. 



Filterability : Fails to pass Berkefeld 

 and Chamberland filters. 



Other properties : May remain viable 

 at least 2 years in dry state. 



Literature : Escherich and Miyajima, 

 Naturwissens. Ztschr. f. Forst- u. Land- 

 wirtschaft, 9, 1911, 381-402; Wachtl and 

 Kornauth, Mitth. a. d. forstl. Versuch- 

 swesen Osterreichs, 16, 1893, 1-38; Wahl, 

 Centralbl. Gesam. Forstw., 35, 1909, 164- 

 172; 212-215; 36, 1910, 377-397; 37, 1911, 

 247-268; 38, 1912, 355-378. 



3. Borrelina reprimens spec. nov. 

 F'rom Latin rcprimere, to restrain. 



Common name : Gypsy-moth wilt virus. 



Host: LYMANTRIIDAE—Porthe- 

 iria dispar (L.), gypsy moth. 



Geographical distribution : United 

 States. 



Induced disease : In gypsy moth cater- 

 pillar, flaccidity, disintegration of tissues, 

 eventual collapse as a watery sack. 

 Death occurs in 13 to 29 (average 21 ) days 

 after infection; caterpillar may remain 

 attached to its support by prolegs; skin 

 ruptures easily. Polyhedral bodies orig- 

 inate in nuclei of the tracheal matrix, 

 hypodermal, fat, and blood cells. 



Transmission : By feeding on contam- 



