1292 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Habitat : It is the causative agent of 

 contagious bovine pleuropneumonia. 

 The disease can be transferred to sheep, 

 goats and water buffaloes, but not to 

 mice, rats, rabbits or other experimental 

 animals. 



2. The organism of agalactia of sheep 

 and goats. (Le microbe d'agalaxie con- 

 tagieuse, Bridr4 and Donatien, Ann. 

 Inst. Past., 39, 1925, 925; Anulomyces 

 agalaxiae Wroblewski, Ann. Inst. Past., 

 47, 1931, 111; Borrelomyces agalactiae 

 Turner, Jour. Path, and Bact., 41, 1935, 

 25; Capromyces agalactiae Sabin, Bact. 

 Rev., 5, 1941, 57.) 



These organisms are very similar to the 

 former organisms in morphology, appear- 

 ance of the cultures and growth require- 

 ments. Usually the growth is less vigor- 

 ous, the colonies remain smaller, and the 

 elements of the cultures are more delicate 

 and less easily visible than those of 

 bovine pleuropneumonia. Characteris- 

 tic crystals develop in the cultures. 



Serologically and immunologically this 

 species is distinct from the bovine spe- 

 cies. 



It is the cause of a systemic disease in 

 sheep and goats with involvement of the 

 joints, eyes, and, in lactating animals, 

 the mammary glands. Other species are 

 not susceptible. 



3. Pleuropneumonia-like organisms in 

 dogs. {Asterococcus canis, Types I and 

 II, Schoentensack, Kitasato Arch. Exp. 

 Med., 11, 1904, 227; 13, 1936, 175; Cano- 

 myces ■pulmonis I and II, Sabin, Bact. 

 Rev., 5, 1941, 57.) 



Both types produce slight uniform 

 opalescence in broth. Type I grows in 

 granules and coarse colonies and is ap- 

 parently pathogenic for dogs. Type II 

 grows in somewhat larger granular 

 colonies. 



They are serologically distinct from 

 each other and from the other members 

 of the pleuropneumonia group. 



The connection of these organisms with 

 distemper is not proven. 



4. Pleuropneumonia-like organisms in 

 rats. Ls (Klieneberger and Steabben, 

 Jour. Hyg., 37, 1937, 143; Jour. Hyg., 40, 

 1940, 223; Murimyces pulmonis Sabin, 

 Bact. Rev., 5, 1941, 57.) 



L4 (Klieneberger, Jour. Hyg., 38,1938, 

 458; Murimyces arthritidis Sabin, loc. 

 cit.) 



The pyogenic virus of Woglom and 

 Warren (Jour. Exp. Med., 68, 1938, 513) 

 and L7 of Findlay, MacKenzie, Mac- 

 Callum and Kleineberger (Lancet, 237, 

 1939, 7) are identical with L4. The 

 organisms isolated from infected joints by 

 Beeuwkes and Collier (Jour. Inf. Dis., 

 70, 1942, 1) and Preston {ibid., 70, 1942, 

 180) probably are identical with L4 but 

 they were not typed serologically. 



The requirements for growth, the ap- 

 pearance of colonies and the morphology 

 are very similar to those of the type 

 strain with the difference that long fila- 

 ments are not observed either in liquid 

 or solid media. 



The strains isolated from rats belong to 

 two serological types. L3 was cultivated 

 from chronic lung abscesses, but the num- 

 ber of strains typed is not sufficient to 

 ascertain that all strains isolated from 

 this source belong to one type. The L3 

 strains are not pathogenic for rats in 

 artificial infection. They produce sup- 

 puration in mice when they are injected 

 with agar. 



L4 which is serologically different from 

 Ls was cultivated from abscesses and 

 spontaneous polyarthritis. It produces 

 polyarthritis both in mice and rats. It 

 is not infectious in monkeys, rabbits and 

 guinea pigs. Both L3 and L4 were recov- 

 ered from the brains of mice kept in the 

 same room with rats. 



According to Klieneberger, L3 usually 

 produces somewhat larger and coarser 

 colonies than L4; L3 grows in broth in 

 small granules, while L4 produces an 

 opalescent growth. 



