BY R. GREIG-SMITH. 



673 



Staphylococci which had grown on the surface of nutrient agar 

 were then tested against washed cocci which had been grown in 

 bouillon. 





Cocci per leucocyte. 





i- 



ii. 



iii. 



iv. 



Bacteria grown on dry agar ... 

 Bacteria grown in bouillon 



8-1-8-6 

 6-S 



3*1 

 32 



3-8 

 3-6 



10-6 ((five days' 

 6-1 \ culture.) 



It is clear that there is something in the bouillon that prevents 

 the relative ingestion of the bacteria grown in it. To see if 

 experiment could throw any light upon the nature of this sub- 

 stance, the following modifications of meat-extract were made, 

 and the phagocytic activities of the leucocytes upon the washed 

 bacteria grown in them were noted. 



Experiment i. Cocci per leucocyte 



Raw meat extract 



The same with 0*5% sodium chloride 



„ 1% peptone 



Neutral bouillon 



Experiment ii. 

 Raw meat extract charged with calcium phosphate 

 Raw meat extract 



Neutral meat extract 



Raw meat extract with 0*5% sodium chloride ... 

 The same neutralised 



„ ,, + 1% peptone ( = bouillon) 



Experiment iii. 

 Raw meat extract 



,, ,, with 0-5% sodium chloride ... 

 Neutral meat extract 



,, ,, with 0*5% sodium chloride 



Experiment iv. 

 Acid bouillon (natural acid=0-l%Na 2 C0 3 ) 

 „ with 0-1% Na 2 C0 3 

 „ 0-2 

 »> ») >> 0*3 ,, 



„ 0-4 „ 



,, 0-6 



6-2 



8-0 

 5-5 

 4-8 



4-4 

 5-1 

 7-7 

 6-2 

 5-6 

 5-6 



3-7 



8-6 

 5-0 



7-8 



7-0 



6-8 



5-5 



11-4 



8-1 



127 



10-5 



