BY R. GREIG-SMITH. 



679 



repeat the count. I cannot but think that in such cases some- 

 thing has happened to the corpuscular suspension which induces 

 an abnormal segregation of the white corpuscles. When haemolysis 

 occurs, the polymorphonuclears become more or less everted; and 

 it may be that a partial haemolysis produces differences in the 

 phagocytic avidity as well as in the segregation during smearing. 

 At one time, a set of experiments will show regular variations; 

 at another time a duplicate set will vary irregularly. This would 

 not occur in routine work, for the tests would always be made 

 in the same manner, but in a work such as this the conditions 

 were being continually altered, and variations were to be expected. 



The segregation of the polymorphonuclears was tested in an 

 experiment which was made for the purpose and in which the 

 total corpuscles were employed. The smears were made with a 

 one and one-quarter inch circle held at an angle which varied with 

 the size of the droplet of corpuscular mixture. As a rule the 

 smear measured 40 mm. from the base to the tip of the wedge, 

 and a line was scratched at a distance of 10 mm. from the tip. 

 Holding the smear as it was made, with the base to the left, ab 

 represents the upper margin from base a to the scratch b, be is 

 the upper margin from the scratch 6 to the tip c, bed is the whole 

 tip bounded by the scratch bd, and de is the lower margin from 

 the scratch d to the base e. 



In the columns, the first numbers are the leucocytes in the 

 portion examined; the second numbers are the cocci per leucocyte 

 or phagocytic indices. 





ah 



be 



bed 



de 





Upper margin, 

 first portion. 



Upper margin, 

 second portion. 



Total tip. 



Lower margin. 



1 



40=10-4 



6S = 10-4 



145= 9-4 



17= 8-8 



2 



21= 7'5 



61= 8-] 



314= 73 



20= 8-8 



3 



11= 9-6 



40=10-2 



66= 8-7 



9= 8-7 



3a 



19= 93 



25= 8-6 



107= 6-5 



50= 91 



4 



9=15-5 



20=13-5 



126 = 11-0 



6=12 8 



5 



35= 90 



40= 8-6 



320= 8-8 



20= 7-9 



6 



14- 9 2 



53 = 10-0 



143= 9-7 



20= 9-3 



