684 OPSONISATION FROM A BACTERIAL POINT OF VIEW, ETC., 



The 'polymorphonuclears of the "Cream" and of the "Separate" 

 — There are undoubtedly differences in the speed of deposition 

 of the polymorphs themselves during centrifugalisation. The 

 more compact and less mobile will deposit first and be found in 

 the lower layers, while the less compact and more mobile ones 

 will occur in the upper layers. The latter will be found chiefly 

 in the " cream," whether they are joggled up from the lower 

 layers in a large tube or are deposited in the upper layers of a 

 small tube. The more mobile phagocytes of the "cream" should 

 give a higher phagocytic index than the less mobile cells of the 

 "separate." Such was found to be the case in the following 

 experiment in which different spreaders were used in making the 

 smears in order to vary the individual tests. 



Phago 



cytes 



from the 



cream. 



separate. 



No. of 

 smear, 



na 

 iib 

 iic 

 iid 

 iie 

 iie 

 iif 



ma 

 iiia 

 iiib 

 iiic 

 iiid 



Kind of spreader, 



Portion of smear|Total count of 

 examined. J50 leucocytes. 



fin. square cover glass. 



fin. square paper. 



l^in. circular cover glass. 



l|in. oiled silk, oblique angle 



l^in. oiled silk, rectangle 



convex cut slide. 



fin. square cover glass. 



n. square cover glass. 



across end 

 across end 

 across end 

 upper margin 

 across tip 

 upper margin 

 lower end 

 across end 



145 

 171 

 169 

 153 

 217 

 126 

 126 

 190 



•166 



across end 

 across end 

 upper margin 

 upper margin 

 upper iriargin 

 across end 

 upper margin 



upper margin 

 across end 

 across end 

 across end 

 acr "",s end 



137 

 172 

 109 

 154 

 204 

 154 

 145 

 154 



137 

 146 

 135 

 133 

 139 



138 



The experiment shows that the "cream" counts are very 

 irregular and have a higher average than the " separate." These 



