BY R. GREIG-SM1TH. 



691 



When the tubes containing the mixed suspensions were allowed 

 to lie upon the bench for 30 minutes and were then smeared and 

 enumerated, the phagocytosis was found to be the same as if the 

 tests had been incubated at 37° for 12£ minutes (glycerin) and 

 for 8 minutes (citrate). 



The influence of time and temperature. — The last experiment 

 led to the making of several to determine the minimum, optimum 

 and maximum temperatures during which phagocytosis occurs. 



Temperature. 



Cocci per leucocyte 

 Duration of phagocytosis in 



minutes. 





5 



10 



20 



3° 

 23° 

 30° 



37° 



00 

 05 

 1-3 



o-o 

 11 



2-2 





 2-2 

 3 6 

 44 



The Influence of Temperature. 



Temperature. 





Cocci per 



leucocyte. 















a 



6 



c 



d 



3° 



0'0(30min.) 



0-0(60 min.' 



— 



— 



10° 







o-o 



— 



— 



12° 



00 



— 



— 





15° 



o-i 



0'3 



— 



— 



18° 



0-3 



— 



— 



— 



20° 



0-4 



09 



— 



— 



22° 



05 



— 



— 



— 



25° 



05 



1-3 



— 



— 



30° 



7 



1-9 



- 



— 



33° 





2-0 



— 



— 



35° 



— 



2 5 



3 5 





36° 



— 



— 



3-8 



— 



37° 



15 



3 4 



4 5 



— 



38° 



— 



3 2 



5-0 



34 



39° 



— 



3 9 



5'3 



4 2 



40° 



1-3 



41 



63 



45 



41° 





3-9 



6 5 



6 4 



42° 



10 



3 2 



61 



54 



45° 



0-7 



— 



— 



— 



47° 



5 



— 



— 



— 



From these experiments, it appears that the phagocytosis 

 below 12° is negligible, but that it is pronounced at higher 



