700 OPSONISATION FROM A BACTERIAL POINT OF VIEW, ETC. 



7. The addition of sugar to bouillon lessens the opsonisability 

 of the bacteria. 



8. Anti-opsonins are present in bouillon cultures. 



9. Staphylococci heated with normal saline at 60° give up an 

 anti-opsonin and become more freely opsonised. 



10. Bacteria grown at 22° and at 37° have an equal capacity 

 for opsonin, if pairs of cells are counted as single cells. 



11. Dilute soda destroys the leucocytes, while dilute phosphoric 

 acid has no action either upon the leucocytes or upon opsonisation; 

 calcium acetate and lactate lessen opsonisation, while the citrate 

 and phosphate have no action. 



12. Marmoreck's antituberculous serum contains latent 

 opsonins among other immune bodies. 



13. Clotting has no influence upon the opsonic content of the 

 blood. 



Regarding opsonic technique, 



1. The labour of counting cannot be lessened by modifications 

 in the suspension fluids or in departures from the usual time and 

 temperature of incubation. 



2. Segregation of the phagocytes occurs in the smears. 



3. The leucocytes that are the last to enter or the first to be 

 expelled from the capillary pipette have a more uniform phago- 

 cytic activity than the others. 



4. By slowly centrifugalising corpuscular suspensions there is 

 obtained a leucocytic " separate " which contains polymorpho- 

 nuclears chiefly; these have a more uniform phagocytic activity 

 than those found in leucocyte " cream." 



5. Phagocytosis occurs during the mixing of the suspensions 

 with the serum. 



6. Phagocytosis begins about 12° and reaches an optimum at 

 41°. 



7. Mixing and smearing should be done as expeditiously as 

 possible when the laboratory temperature is over 12 p . 



8. Opsonic determinations should always be made in exactly 

 the same manner in every detail. 



[Printed off November 18th, 1908]. 



