42 The Philippine Journal of Science 1917 



the above case for an antiseptic. The flasks containing the solu- 

 tions were incubated at 39° C. 



In view of the hydrolyzing effect of crab juice it was thought 

 that it might prove interesting to determine the effect of adding 

 blood to similar samples. 



Table IV. — Action of blood on a 1 per cent solution of gynocardin. 



Time. 



Kind of blood. 



Blood. 



Gluco- 

 Bide. 



Gluco- 



hydro- 

 lyzed. 



Hours. 

 21.5 

 21.5 

 22.5 

 45.5 

 45.5 

 46.0 



Blood from apparently healthy person 



6 



3 

 6 



cc. 

 25 

 25 

 25 

 25 

 25 

 25 



Per cent. 

 1.45 

 1.69 

 3.63 

 3.15 

 6.14 

 3.96 













« This blood was kindly furnished me by Dr. J. W. Smith, in charge of Bilibid Prison. 



The above results indicate that blood from tubercular pa- 

 tients reacts more rapidly with gynocardin than does the blood 

 of the normal person. The blood from the syphilitic person 

 apparently reacted more rapidly the first day, but the sample 

 incubated two days reacted no faster than the sample from the 

 normal person. The Bureau of Science has collected some 

 further data concerning this property when amygdalin is used 

 that substantiates the above evidence. 



Amygdalin has been found to be harmless when taken into 

 the system unless administered in the presence of emulsin. Gy- 

 nocardin, when given in doses of 0.25 and 1 gram to guinea 

 pigs, was found to be without any apparent effect. At the end 

 of twenty-four hours no further observations were taken, as the 

 pigs appeared perfectly normal. 



GYNOCAKDASE 



The enzyme gynocardase was obtained from the leaves of 

 Pangium edule by grinding the leaves very fine and then pressing 

 in a hydraulic press, adding an equal volume of alcohol to the 

 juice and filtering. The precipitate was then ground under 

 water with sand, filtered through cloth, and again precipitated 

 by the addition of alcohol. The precipitate was nearly black 



