188 The Philippine Journal of Science uh 



and ethyl acetate. When thought necessary, these extractions 

 were on material made acid or alkaline. The extracts freed from 

 the solvent produced no physiological effects on dogs or guinea 

 pigs. In no case was any disturbance in the activity of guinea 

 pigs observed from the administration of any portion of this 

 plant. These results confirm Renson's conclusions regarding the 

 nonpoisonous character of the plant toward the Herbivora. 



After the extractions with organic solvents neutral warm 

 water was used to extract the plant. Vacuum evaporation of 

 the filtered solution gave a small mass of crystalline substance 

 which was recrystallized once from methyl alcohol followed by 

 ethyl alcohol. The quantity was insufficient for analytical pur- 

 poses. Eight milligrams were given a healthy 1-year-old dog. 

 The compound exhibited the same effect as that produced by 

 the crude drug. In this case the animal showed a change in 

 mental condition similar to that observed by Renson 88 in his 

 "second form of poisoning." Although the plant material con- 

 goura (Rourea oblongifolia) used by Renson is not the same as 

 that used by us, yet our results indicate that these plants contain 

 the same active principle. He describes what he terms the 

 "second form of poisoning" in the above reference as follows : 

 First or Acute Form of Poisoning, followed by Death. 



Two or three hours after the hypodermic injection the animal commences 

 to vomit without great effort, it expels fecal matters, and trembles a little. 

 An increase of activity in the salivary glands is than observed, a clear and 

 limpid liquid trickling from the mouth, drop by drop and in quantity rela- 

 tively great, as though jaborandi had been taken. Soon the movements in 

 walking become uncertain, the animal stumbles, and appears intoxicated. 

 Later it remains lying down and there is a loss of voluntary movements. At 

 last convulsions occur, the mouth remains open, and the pupils are dilated. 

 These convulsions are accompanied by moans and blinking of the eyelids. 

 Then appears a most curious phenomenon. The dog, although lying on its 

 side, executes the movements of walking. With ears and tail erect, it shakes 

 its head in an almost lively manner, and there is no doubt it believes it 

 walks. This dream, if I may so call it, immediately follows the convulsive 

 attack. The attacks are repeated a great number of times, and in the 

 intervals there are hiccups and sudden starts. At last the animal, com- 

 pletely worn out, falls into a comatose state, its respiration becomes un- 

 equal, it emits a little blood by the nostrils (in certain cases, but not in 

 others), and dies at the end of ten to twelve hours. 



Second Form of Poisoning, followed by Recovery. 



It suffices to introduce half a cubic centimeter of the liquid extract of 

 Cangoura into the organism, either by means of the skin or the stomach, to 

 produce the following effects in a dog of average size. The day of injection 



" Loc. cit. 



