200 



The Philippine Journal of Science 



kaloids from acid solutions. 3 This property of infusorial earth 

 caused Seidell ' to attempt the extraction of vitamine from brew- 

 er's yeast.'' The above-named investigator obtained yeast from 

 the breweries and subjected it to pressure to free it from the beer 

 retained in it. The yeast was then autolyzed at a temperature 

 of 37.5° C. and filtered. The brown filtrate was active, protecting 

 a pigeon from the onset of polyneuritis for several months when 

 administered in doses of 1 cubic centimeter on alternate days. 

 This active liquid was treated with a prepared hydrous aluminium 

 silicate (Lloyd's reagent) in the proportions and with the results 

 given in Table I. 



Table I. — Extraction of yeast liquid with Lloyd's reagent. 



Quan- 

 tity of 

 yeast 

 liquid. 



Quan- 

 tity of 

 Lloyd's 

 reagent. 



Activity of filtrate. 



Activity 

 of extract. 



cc. 



1.000 

 1,000 

 1,000 

 1.000 



O. 



200 

 20 

 40 

 50 





Active. 

 Do. 







Do. 





Do. 







Twenty grams of Lloyd's reagent were insufficient to extract 

 all the vitamine from a liter of yeast liquid ; 40 grams left a small 

 proportion remaining in the filtrate. After extraction with 50 

 grams of Lloyd's reagent, the filtrate possessed only slightly pro- 

 tective powers from polyneuritis when administered to pigeons. 



EXPERIMENTAL PART 



Because of the difficulty experienced in keeping the extract of 

 rice polishings sterile, it was decided to investigate the possi- 

 bility of extracting the hydrolyzed extract of rice polishings 

 with infusorial earth. This infusorial earth was obtained from 

 Japan. Its absorptive powers are less than those of Lloyd's 

 reagent. Under the conditions described by Lloyd for testing 

 the efficiency of his reagent, Japanese infusorial earth reacted 

 as follows: 



3 Lloyd, John U., Journ. Am. Pharm. Assoc. (1916), 5, 381. 



'Seidell, Atherton, U. S. Pub. Health Rep. (1916), 31, 364. 



6 Brewer's yeast contains the highest percentage of any of the known 

 sources of vitamine. As it is a waste product in the breweries, it is also 

 a cheap source of vitamine. 



