Brill: Infusorial Earth Extract 



201 



Table II. — Absorptive power of Japanese infusorial earth for morphine 

 bisulphate.* 



Quantity 



of in- 

 fusorial 

 earth 

 used. 



Result with Mayer's reagent 

 on the filtrate. 



a- 



0.60 

 0.40 

 0.35 

 0.30 

 0.25 



Not opalescent. 

 Slightly opalescent. 



Do. 



Do. 

 Strongly opalescent. 



° Five cubic centimeters of a 1 per cent solution of morphine sulphate diluted to 80 cubic 

 centimeters. Lloyd reports his reagent to have an absorptive power of 8 grams of earth 

 to 1 gram of morphine bisulphate. This is nearly the same proportion given by the Japanese 

 earth. The latter has an absorptive power of 10 grams of earth to 1 gram of morphine 

 bisulphate. 



The alcoholic extract c of rice polishings was hydrolyzed by 

 heating with sulphuric acid in the manner described by Williams 

 and Saleeby. 7 One 500 cubic centimeter portion of this hydro- 

 lyzed extract was treated with 50 grams of the Japanese in- 

 fusorial earth with shaking at intervals for a period of one hour. 

 The mixture was allowed to subside, and the supernatant liquid 

 was siphoned off. The infusorial earth was then placed on a 

 suction funnel and washed with a 5 per cent sulphuric acid 

 solution and finally dried. A second 500 cubic centimeters por- 

 tion was treated with 25 grams of infusorial earth in a similar 

 manner. The extracts, when dry, were placed in capsules for 

 administration. The 50-gram sample, hereafter referred to 

 as D, weighed 95 grams and filled 174 capsules, or each 

 capsule represented the extract from 2.9 cubic centimeters of 

 solution. The 25-gram sample, hereafter referred to as E, 

 weighed 49 grams and was placed in 135 capsules, or each capsule 

 represented the extract from 3.7 cubic centimeters of solution. 

 The filtrate from D was bottled, subjected to fractional steriliza- 

 tion, and marked B; the filtrate from E was bottled, subjected to 

 fractional sterilization, and marked C. The original hydrolyzed 



' This extract is made by treating 25 kilograms of rice polishings with 

 5 demijohns of 25 per cent alcohol and later pressing this. The extract is 

 evaporated at a low temperature, the fat is separated, and alcohol is added 

 to precipitate the albuminous and other matter, and again the extract is 

 evaporated. The solution is concentrated, so that 1 cubic centimeter of 

 extract is equivalent to 15 grams of rice polishings. 



'Williams, R. R.. and Saleeby, N. M., This Journal, Sec. B (1915), 10, 

 106. 



