204 



The Philippine Journal of Science 



Of the three chickens treated one showed signs of polyneuritis 

 at the end of one hundred one days, the second at the end of 

 twenty-five days, while the third was still healthy at the end of 

 one hundred eighty-six days. The vitamine has not been entirely 

 extracted, as B still has protective properties. 



In the comparison made on morphine bisulphate, the Japanese 

 infusorial earth compared favorably in absorptive powers with 

 Lloyd's reagent, but Seidell found that 50 grams of Lloyd's re- 

 agent extracted all the vitamine from 1 liter of autolyzed yeast 

 liquid, while in the above experiment 50 grams of infusorial 

 earth have not extracted all the vitamine from 500 cubic centi- 

 meters of extract. The difference in the absorption must be due 

 to the difference in the character of the two solutions. Hydro- 

 lyzed extract of rice polishings is a thick, syruplike liquid, which 

 undoubtedly collects on the infusorial earth and renders it in- 

 capable of completely extracting the vitamine from the extract, 

 while the yeast extract is no more viscous than water. 



When 500 cubic centimeters of extract of rice polishings are 

 treated with 25 grams of Japanese infusorial earth, the filtrate, 

 C, still has protective properties similar to those possessed by B. 



Table VI.— History of chickens treated with 3 cubic centimeters of C on 

 alternate days (three times weekly). 



No. of 

 chick- 

 en. 



Days 



before 

 onset of 

 poly- 

 neu- 

 ritis. 



63 

 63 



Total 

 number 

 of days 

 on pol- 

 ished 

 rice. 



186 



68 



n. 



Treatment. 



Num- 

 ber of 

 treat- 

 ments. 



Total 



loss in 

 weight. 



Results and remarks. 



1 

 2 



1 capsule of E on alter- 

 nate days. 



1 



none 



P.ct. 

 13 



41 



16 



Alive at end of experiment. 



Improved, but died four 



days later. 

 In healthy condition at end 



of experiment. 







The infusorial earth extracts D and E possess antineuritic 

 properties. The chickens, No. 1, of Table V, and Nos. 1 and 2, 

 of Table VII, were benefited by the administration of D when 

 they had contracted polyneuritis. Chickens 1 and 2, of Table 

 VI, and 1, of Table VIII, were benefited by treatment with E. 



To test the activity of the infusorial earth extracts, the ex- 

 periments described in Tables VII and VIII were performed. 



