214 The Philippine Journal of Science 1917 



Table VII. — Iodine value of acids isolated from chaulmoogra oil. 



Sample. 



Quantity. 



0.1 A' 

 iodine 

 solution 

 used. 



Iodine 

 value 

 (Han- 



U3).» 



Chaulmoogric acid: 



a- 



0.1806 

 0. 1558 



0. 2250 

 0. 1625 



12.73 

 11.13 



17.71 

 12.73 



89.5 

 90.7 



100.2 

 99.9 





Hydnocarpic acid: 



::::::;::::;;;:;:: :;::;: :::;;:::::::: .:::::::: 



i 



1 Theoretical iodine value for the addition of two atoms of iodine to chaulmoogric acid, 

 Ci 7 H 3l COOH = 90.6 ; for the addition of two atoms of iodine to hydnocarpic acid. C15H07COOH 

 = 100.6 



Preparation and description of samples. — A sample of chaul- 

 moogra oil was washed with three separate portions of alcohol. 

 The alcohol took on the color of the chaulmoogra oil, and a par- 

 tial solution of the two took place. The separation was effected 

 by adding water to the mixture, when the alcohol and oil formed 

 separate layers. The alcohol-washed oil had the properties 

 noted in Table VIII. 



Table VIII. — Properties of chaulmoogra oil after washing with alcohol 



Specific gravity 0.9530 



Specific rotation in chloroform for sodium light +52.11 



Acid value cc. 0.1 N base 2.46 



Saponification value 196.3 



Index of refraction 1.4735 



The alcohol extract after extraction with petroleum ether was 

 distilled under lowered pressure until all the water and alcohol 

 were removed and only a small residue remained; it was then 

 dried over sulphuric acid in a vacuum desiccator. The residue 

 is a golden yellow, viscous, almost odorless, noncrystallizable 

 mass. It constitutes about 0.06 per cent of the original oil. It 

 was insoluble in olive oil, but was suspended in 50 cubic centi- 

 meters of this oil, marked No. 1, and submitted to the authorities 

 at Culion with the following directions: 



Directions: 1 cubic centimeter = 80 cubic centimeters chaulmoogra oil; initial 

 dose 0.10 cubic centimeter increasing to 0.50 cubic centimeter; shake 

 before using. 



The sample used in this manner at no time caused a reaction, 

 that is, it appeared to be physiologically inactive when given 

 in the above doses. The petroleum ether extract of the alcohol 

 extract was placed on the steam bath for the removal of the pe- 

 troleum ether and finally dried in a vacuum desiccator. This oil, 



