Brill and Williams: A Specific for Leprosy 



215 



marked No. 2, is darker than No. 3 (described later) ; it is solid 

 at ordinary temperature (30° C). It constituted about 26.2 per 

 cent of the original oil. 



Directions: Dose same as chaulmoogra. 



The alcohol-washed oil was then placed in a cold room (tem- 

 perature, 17" to 19° C.) for four days, where it hardened to a 

 viscous mass. When placed in cheesecloth and hung, only a 

 very small portion separated. It was then placed in a hand 

 press and subjected to pressure. The fractions resulting were 

 again subjected to fractionation by means of the press, and the 

 hard fractions were mixed, and the soft samples that resulted 

 were mixed. The soft oil fraction, known as No. 4, was the 

 darkest of the three samples. It is soft at 17° C. and constitutes 

 about 20.7 per cent of the original oil. 



The hard oil fraction, submitted to Culion as No. 3, is darker 

 than No. 2, but not so dark as No. 4. It is hard at 17° C. and 

 constitutes about 53 per cent of the original oil. The directions 

 for Nos. 3 and 4 were identical with the directions for No. 2. 



The chemical constants of these three fractions are tabulated 

 in Table IX. 



Table IX.— Constants of fractions Nos. 1, 2, and 3. 



Sample No. 



! Specific 

 c, !a _ ' rotation 



at du" u. sodjum 

 light. 



Acid va- 



luein cc. 



0.1 N 



base. 



Saponifi- 

 cation 

 value. 



Index of 

 refrac- 

 tion. 



Iodine 

 value. 



Original 

 oil. 



1 

 Remarks. 



2. 



0.9440. +52.35 

 0.6522 1 +55.03 

 0.9537 -54.84 



17.08 



190.2 

 194.4 

 191.3 



1.4699 





P. cent. 

 26.2 



Solid at 30° C. 

 Solid at 17° C. 

 Liquid at 17° C. 







4 





1.4764 



103.4 



20.7 



None of the fractions showed any greater activity than the orig- 

 inal chaulmoogra oil, that is, no more marked reactions were ob- 

 served than are caused by the administration of chaulmoogra oil. 



Sample 2, Table II, which was obtained by petroleum ether 

 extraction of seeds received from Madras from H. G. Carter, 

 economic botanist to the Botanical Survey of India, and listed 

 as seeds of chaulmoogra, was forwarded as No. 5. Its proper- 

 ties place it with chaulmoogra oil. Directions: Dose same as 

 chaulmoogra oil. The sample gave no reaction, differing in in- 

 tensity from that of ordinary chaulmoogra oil when it was ad- 

 ministered. It seemed no less active than many commercial 

 samples used by the Public Health Service. 



