﻿VITAMIN B IN EDIBLE TISSUES OF OX, SHEEP, AND EOG. 35 



40 minutes at 210°-220° C. The cooked heart was then ground and 

 dried. Pen 64 was fed a ration containing 15 per cent of the cooked 

 ox heart. Two pigeons developed polyneuritis, one on the twenty- 

 second day, the other on the thirty-seventh day, and the other two 

 buds were in good condition at the close of the test on the fifty-ninth 

 day. By comparing the result of this test with that secured from 

 feeding the ration that contained 15 per cent of uncooked ox heart, 

 pen 45, Table 16, it is evident that the method employed in cooking 

 the ox heart materially lowered its antineuritic value. 



The ox kidney was cooked in the following manner: The fresh kid- 

 neys were split open, trimmed as free as practicable from fat and con- 

 nective tissue, cut into small cubes, and then washed thoroughly in 

 cold water. The kidney was then placed in a kettle, covered with 

 water, and heated to boiling. The water was poured off, fresh water 

 was added, and boiling was continued for an hour when the kidney 

 was sufficiently tender to eat. The water was poured off and the 

 cooked kidney was ground and dried is the usual manner. Pens 65 

 and 66 were fed rations which contained 15 and 25 per cent, respec- 

 tively, of the cooked ox kidney. The ration containing 15 per cent 

 of the cooked ox kidney had a rather low antineuritic value. Three 

 pigeons developed polyneuritis on the twentieth, twenty-fourth, and 

 fiftieth days, respectively, and one bird was in very poor condition at 

 the close of the test on the fifty-sixth day. The ration that contained 

 25 per cent of the cooked kidney had a fair antineuritic value. One 

 pigeon developed polyneuritis on the fifty-fourth day; the three others 

 were in fair condition at the close of the test on the fifty-sixth day. 

 The pen of birds suffered an average loss in weight of 13 per cent, 

 which is an indication of a deficiency of the antineuritic vitamin in 

 the ration. 



TESTS WITH OX BRAINS AND LAMB BRAINS. 



The results of the feeding test with ox and lamb brains are reported 

 in Table 22. The ration containing 15 per cent of ox brains had a 

 very low antineuritic value, three of the birds having developed 

 polyneuritis by the nineteenth day and the fourth bird was in poor 

 condition at the close of the test period of 55 days. The average 

 survival period was 26 days and the loss in weight 9.7 per cent. 

 The ration which contained 25 per cent of ox brains had a fair anti- 

 neuritic value, the average survival period being 41 days and the loss 

 in weight 6.7 per cent. Two birds developed polyneuritis on the 

 twenty-fifth and thirtieth days, respectively; the other two were in 

 good condition at the close of the test. 



Lamb brains had practically the same antineuritic value as ox 

 brains. One bird in pen 70 died on the twenty-second day of the 

 test on account of an injury. The changes in the weights of the 

 pigeons getting the ox-and-lamb brains ration are shown in Figures 

 35 and 36. 



