PLATE-L. 

 Effects of Abnormal Temperature. 



Fig. 94. Exp. 1170 (1): Ca. 38° C. 1 hr.; egg irregular in outline, with archiplasm withdrawn into 

 amphiaster, and into the surface layer. First maturation amphiaster irregular in shape and chromosomes 

 scattered; sperm nucleus near vegetal pole. 



Fig. 95. Exp. 1174 (2) : 37° C. I hr., room temp. (27°) 3 hrs. ; first polar body very large; chromosomes 

 of second maturation division have formed karyomeres; sperm nucleus near animal pole. 



Fig. 96. Exp. 1171 (1): Ca. 35° C. i hr.; 2-cell stage, showing dense aggregation of archiplasm 

 around nuclei and spheres, with different kinds of cytoplasm in other parts of cell. 



Fig. 97. Exp. 1171 (1) : Similar to preceding; second cleavage spindles greatly modified; chromosomes 

 scattered; archiplasm gathered in spindle areas and division wall. 



Fig. 98. Exp. 1171 (2): Ca. 35° C. J hr., room temp. (ca. 24°-26°) 15 hrs.; the archiplasm has col- 

 lected in central areas in each cell and in division walls; chromosomes are clumped and thrown out of cyto- 

 plasmic areas. 



Fig. 99. Exp. 1171 (2): Similar to preceding. 



Fig. 100. Exp. 962: On ice 16 hrs.; the spheres of the third cleavage are unusually distinct and the 

 scattered sphere granules of the second cleavage (in the micromeres) are very large. 



Fig. 101. Exp. 962: The spheres have a definite boundary, stain more deeply than usual and look 

 almost like nuclei. 



Fig. 102. Exp. 962: A later stage, with spheres similar to those shown in fig. 101. 



Fig. 103. Exp. 964: On ice 40 hrs.; the sphere granules are especially large. 



Fig. 104. Exp. 964: Similar to preceding. 



Fig. 105. Exp. 964: Similar to preceding; many of the sphere granules are vesicular. 



