I am indebted to Mr. Griswold for the following notes, 

 which by their minute detail, show the care which has 

 been used; as has been stated before, the scheme of work 

 .was arranged by Mr. James W. Milner, Deputy Com- 

 missioner of Fisheries. 



At 5 a. m. on the 15th inst., 45,000 shad, j>ist out of 

 the eggs, were taken from the hatching boxes at Holy- 

 'oke, Mass. , and placed in five forty-quart tin cans for 

 transportation. 



Noank was reached at 11:30 the same morning; dur- 

 ing the transit and for ten hours after theii arrival, the 

 fish were supplied with tresh spring water every two 

 hours; one-eighth of the total amount of water in the 

 cans being drawn off and replaced by new. At 9 p. m. 

 the experiment was inaugurated, the fish being placed 

 in ten four gallon stone jars — about four thousand in 

 each. The jars were filled with fresh spring water 

 which was to be gradually replaced by sea water until a 

 complete change was made. Four schemes were simul- 

 taneously begun, the details of which will be given in 

 proper order; in these it was the ai i; to keep the tem- 

 perature uniform with that of the water in the bay. It 

 should be stated that the water in the jars, after the 

 completion of the schemes of change,- cannot have been 

 pure salt water, but yet so nearty approximated it that 

 there was probably no appreciable difference. The 

 water was changed every third hour, two quarts or one- 

 eighth of the old water being drawn off by a rubber 

 siphon, and replaced by a new supply with which salt 

 water was added in a regular ratio ot arithmetical pro- 

 gression which varied in the different schemes. The 

 fresh water was brought from a spring half a mile dis- 

 tant, and this as well as the salt water, had the advan- 

 tage of being dipped up, not drawn through a slimy 

 pump. The utmost care was taken to preserve the clean- 

 liness of the jars. 



Scheme I. — (Jar No. 1): To change the water from 

 fresh spring water to sea water in 45 hours; adding one 

 gill of sea water at each change. Thus at twelve, mid- 

 night, two quarts of the old water was withdrawn and 

 replaced by a mixture of fifteen gills of spring water 

 and one of sea water; at 3 a. m., fourteen gills of 

 spring water and two of sea water, and so on. This 

 scheme was completed on the 17th, at 6 p. m. The tem- 

 perature of the water ranged from 69° to 71°. On the 

 18th at 9 a. m. the young shad showed signs of weak- 

 ness, and at 6 p. m. were all apparently dead, 85 hours 

 after leaving the hatching boxes, and 24 hours after the 

 water ban become entirely salt. 



Some were taken at the last moment from the bot- 

 tom of the jar, where they had fallen, and placed in a 

 two quart glass jar (.Jar ifo. 11), filled with a mixture of 

 half gait aacl half spring water; h,er§ they revived, &£$ 



