96  Self-Incompatibility in Hermaphrodite Plants 
single anther sac was observed to dehisce, and repeated examinations of 
the contents showed that at least 95°/, of the pollen was shrivelled and 
empty. On three other plants, a few anthers were observed to dehisce 
partially, but in these the pollen was likewise largely impotent. All 
these plants produced corollas that opened normally, and every one 
produced large capsules with seed to free open cross-pollination. These 
plants exhibited a one-sided impotence identical with that observed 
in various species, such as in certain so-called self-sterile varieties of 
cultivated grapes and of the navel orange. 
Twelve plants were fully potent both as males and females. They 
bloomed from 16 to 28 days, and controlled self-pollinations were made 
for from 20 to 64 flowers per plant. Five were completely self-incom- 
patible, two were partially self-fertile, and five were highly self-compatible. 
The latter gave fine large pods in nearly every flower from the first to 
the very last to bloom. Of the partially self-compatible plants, one 
produced pods only on first two dates of selfing, and the others produced 
numerous but small pods indiscriminately throughout the period of 
loom. : 
Summary. There was no evidence of “end-season” self-fertility in 
any of these plants. Plants were strongly or feebly self-fertile from the 
first flower that opened, and all plants completely self-incompatible 
during the first part of the blooming period remained thus to the end, 
In some cases of feeble self-fertility the self-fertility appeared to be 
confined to the early and mid-season period of bloom. 
Eschscholtzia californica. 
For the seed of this species the writer is indebted to Professor 
W. T. Horne, who collected it from individual plants growing wild on or 
near the campus of the University of California. Seeds of each of 
5 plants were sown separately during March, 1918. All but three of 
the plants were grown in pots in a greenhouse. Flowers were enclosed 
in glassine bags and allowed to make autonomous self-pollination, a 
procedure that ‘supplies an abundance of pollen to stigmas at a time 
when they are receptive to compatible cross-pollinations. The dehiscence 
of the stamens of individual flowers continues for several days, during 
which time the stigmatic branches of the pistil elongate, coil about 
among the stamens and become covered with pollen. Pollen and 
stigmas in this stage used in crossing most often give a rapid develop- - 
ment of fruit and seeds, : . 
