Estimation of Organic Nitrogen. 399 



CH 3 -CN + HC1 + 2H 2 > CH 3 -COOH + NH 4 Cl 



acetonitrile 



C 2 H-CN+HC1 + 2H 2 > C 2 H 5 -COOH + NH 4 Cl 



propionitrile 



O 



O.H.-C-NH, + HC1 + H 2 > C 2 H -COOH + NH 4 C1 



propionamidie 



DH a -CCX CH 2 -COOH 



)NH + HC1 + 2H 2 >\ +NH 4 C1 



CH 2 -CO/ CH-COOH 



succinimide 



/COOH 



CN-CH 2 -COOC 2 H 6 + HC1 + 3H 2 > CH/ + NH 4 C1 + C 2 H 5 OH 



2yan-acetic ester ^COOH 



CN H,COOH 



DH 3 -CH-COOC 2 H 6 + HC1 + 3H 2 > OH 3 -c( + NH 4 C1 + C a H 5 OH 



x-cyan-propionic ester COOH 



CN H y COOH 



D a H B -CH-COOC 2 H 5 + HCl + 3H 2 > C„H,c( + NH 4 C1 + C 2 H 5 OH 



i-cyan -butyric ester COOH 



3H-CLI 2 -CH CN C 8 H COOH + NH 4 C1 



)C-COOC 2 H 5 4-HCU3H 2 > )C( 



DH -CH.-CH/ C,h/ \COOH + C 2 H 5 OH 



a-dipropyl a-cyan-acetic ester 



NH 2 -CH 2 -COOH + HCl + H a O > H0-CH 2 -C00H + NH 4 C1 



imino-acetic acid 



of hydrochloric acid of about 1*2 sp. gr. were added through a 

 long-stemmed funnel. The tube was then sealed, cooled, and 

 in the case of liquid substances the capillary bulb was broken 

 by giving the tube a few smart raps on the palm of the hand. 

 The sealed tube was now inserted in an iron casing and the 

 whole placed in a Carius combustion furnace. The temperature 

 was raised to between 180° and 215°, preferably to about 200°, 

 and kept at this point for two to two and a half hours. In the 

 case of glycocoll a longer time was found necessary for complete 

 decomposition. After the combustion was considered to be com- 

 plete the tube was cooled, the end of the tube was fused to 

 relieve any pressure resulting from the decomposition of the sub- 

 stance, after which the tube was entirely removed from its case, 

 the end cut off and the contents washed into an evaporating 

 dish without loss. In order to remove the excess of hydrochlo- 

 ric acid the solution was evaporated to dryness on a steam or 

 water bath under a draught hood, leaving the evaporating dish 



