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74 Pond.—The Incapacity of the 
Demonstration of tannin in the endocarp. The thin membranous 
endocarp may be obtained in quantity as a chaff by sifting the powder, 
especially after the first time through the mill. On boiling some of this 
endocarp chaff in water, a light brown solution was obtained, which gave 
with ferric chloride a brown precipitate and a brownish green filtrate. By 
using a more dilute solution of the reagent a typical blue green colour 
without a precipitate was obtained, On adding lead acetate a grey pre 
cipitate fell leaving a water clear filtrate. Potassium ferrocyanide caused 
a grey precipitate to fall. Iodine gave a reddish brown precipitate. 
A cold aqueous extract of the endocarp chaff gave a typical reduction 
of Fehling’s solution, probably due to the reducing sugar held by the 
membranes. Another portion of chaff, after being washed until an hour's 
aqueous extract at ordinary temperature did not reduce Fehling’s solution, 
was extracted with boiling water. This extract reduced Fehling’s solu- 
tion, presumably because of the tannin rather than because of sugaf. 
That tannin was present was made certain by the test with ferric chloride. 
Further, this extract gave the same colour reaction with alkali as the 
powdered endosperm from which the tannin was not obtained after long 
digestion in cold water. The endosperm substance entirely freed from 
endocarp substance by scraping the dry seed does not develop this colour 
with alkali. Thus it seems probable that the endosperm powder prepared 
as previously described above contains so little tannin or associated 
substances that not enough goes into solution in cold water to react with t 
ferric chloride, but that enough is present to produce the change noted 
when alkali is added. 
Recapitulation of preceding data. The facts of special interest now 
revealed are: the endosperm contains reducing sugar, the endocarp con- 
tains tannin, the only proteid present in significant amount is the nucleo- 
proteid. 
Experiment No. \. 
auto-hydrolytic? For this test 400 grms, of powdered endosperm were 
aie 2 per cent.) and reprecipitated by slight acidification with dilute acetic acid. This process e 
trates were amber coloured. The final precipitate seemed 48 
onducted three 
resembling the original precipitate remained, containing phosphorus. 
alkali and appeared to be a nuclein or a mixture containing it. Hydrated with 2 per cent. HCL 
a deep red solution was formed, and a brownish black, flocculent material collected. The latter 45 
slowly soluble in dilute alkali and, after being ong agg eg acid, the colour remained the 
same and no effort was made to determine aie nature of the la 
alkaline with NH,OH. Typetinees. © with an 
bell 
E g twenty four hours. These observa 
at: = Bie es + aL 4al . Loe 7 Lo = - 
s, 
= se P bst t contains not only nucleoprotsil 4 
y noted. It is is possible also that other substances 
or com- — 
so of Fnsopr are eet ad further study with more material would be necessary t? 
: 
Is the aqueous extract of the resting endosperm _ 
It was soluble in dilute 
The filtrate was made strongly : 
srsmoniscal silver solution had no effech 
Syn s 
RRR SPS Se EE RE me re ae AEE 
pK pss 
Syed ie ena ae 
a 
i a 
