Solution Tension and Toxicity in Lipolysis.  . 277 
TABLE XIII (continued), 
4096 0.05 0.15 1.00 0.85 
2048 0.10 0.20 1.00 0.80 
1024 0.20 0.30 1.00 0.79 
512 0.45 0.50 1.00 0.50 
256 0.90 0.90 1.40 a 
128 1.70 1.80 2.10 0539 
64 3-40 3-40 3-60 m80 
32 . ae 6.70 6.70 0.00 
Water : O.1 1.10 0.95 
Enzyme, 0.125 per cent. Incubation period, 4 hours. 407: G. 
This shows that 32 is the toxic limit under the conditions of the 
test. There is only slight inhibition at 8192, and none at 16384- 
Notable is the fact that, while the enzyme has an initial acidity 
of 0.15, and the control also has this value of acidity in the concen- 
trations of the reagent having no acidity, as the acidity of the 
reagent increases by concentration, the acidity of the control and 
reagent become more nearly equal, and finally are the same. This 
is interesting in connection with the results of tests soon to be given 
here, which show that the toxicity of the metals tends toward equality 
with increasing concentration of the enzyme. The following table 
shows that there is a reaction between the toxic agent and the sub- 
stance of the enzyme solution. In this test the ethylbutyrate was 
omitted. 
TABLE XIV. 
ZINC. 
M. Reagent. Boiled. Unboiled. 
32768 0.00 0.15 0.25 
16384 0.00 0.15 O45 
8192 0.00 0.15 0-25 
4096 0.05 0.15 0.25 
2048 0.10 0.20 0.28 
1024 : 0.20 0.25 0.36 
512 RAS 5) 0.50 OG 
256 Ob0 4 2, 0.85 0.85 
128 1.70 Oe see te 
64 3-40 3.40 3-49 
Water 
0.15 0139 
Enzyme, 0.25 per cent. recuse period, 4 hours. 40° C. Initial acidity 
of the boiled and unboiled enzyme solution with toxic agent absent a” 
— incubation = 0.15. 
