§ 293. Cataloging Preparations. — It is believed from personal ex- 

 perience, and from the experience of others, that each preparation 

 (each slide or each series) should be accompanied by a catalog contain- 

 ing at least the information suggested in the following formula : 



A Catalog- Card Written According to 

 this Formula : 



Muscular Fibers. Cat. 



C. 15. 

 Fibers 20 to 40 /.i thick.. 



2. No. 475. (Drr. IX) Oct. 1, 1891. $. 

 H. G. , Preparator. 



3. Tendinous and intra-muscular ter- 

 minations of striated muscular fibers 

 from the Sartorius of the cat {Felis do- 

 mestica. ) 



4. Cat eight months old, healthy and 

 well nourished. Fasting and quiet for 

 12 hours. 



5. Muscle pinned on cork with vas- 

 elined pins and placed in 20 per cent, 

 nitric acid immediately after death by 

 chloroform. Left 36 honrs in the acid ; 

 temperature 20 C. In alum water (% 

 sat. aq. sol.) 1 day. 



6. Fibers separated on the slide with 

 needles, Oct. 3. 



7. Stained 5 minutes with Delafield's 

 hematoxylin. 



8. Dehydrated with 95%. alcohol 5 

 minutes, cleared 5 minutes with carbol- 

 turpentine, mounted in xylene balsam ; 

 sealed with shellac. 



9. Use 18 mm. for the general appear- 

 ance of the fibers, then 2 or 3 mm. ob- 

 jective for the details of structure. Try 

 the micro-polariscope (J 209). 



10. The nuclei or muscle corpuscles 

 are very large and numerous ; many of 

 the intra-muscular ends are branched. 

 See S. P. Gage, Proc. Amer. Micr. Sci., 

 1890, p. 132 ; Ref. Hand-book Med., Sci., 

 Vol. V., p. so- 



il 294. General Formula for Catalog- 

 ing Microscopical Preparations : 



1. The general name and source. 

 Thickness of cover glass and of sections. 



2. The number of the preparation and 

 the date of obtaining and fixing the 

 specimen ; the name of the preparator. 



3. The special name of the prepara- 

 tion and the common and scientific 

 name of the object from which it is de- 

 rived. Purpose of the preparation. 



4. The age and condition of the object 

 from which the preparation is derived. 

 Condition of rest or activity ; fasting 

 or full fed at the time of death. 



5. The chemical treatment, — the 

 method of fixing, hardening, dissociating 

 etc., and the time required. 



6. The mechanical treatment, — im- 

 bedded, sectioned, dissected with nee- 

 dles, etc. Date at which done. 



7. The staining agent or agents and 

 the time required for staining. 



8. Dehydrating and clearing agent, 

 mounting medium, cement used for 

 sealing. 



9. The objectives and other accesso- 

 ries (micro-spectroscope, polarizer, etc. ) 

 for studying the preparation. 



10. Remarks, including references to 

 original papers, or to good figures and 

 descriptions in books. 



LABELING PREPARATIONS. 



(1) The number of the preparations, the 



thickness of the cover-glass and 

 of the sections under it. 



(2) The name and source of the prepara- 



tion. 



(3) The date of the specimen ( 2 of 



catalog. ) 



No. 475- 



C. .js_ 

 Sees. Sju 



Striated Muscle ; transection of the 

 Sartorius of the Cat. 



October 15, 1894. 



