CH. X] 



PARAFFIN METHOD 



3°3 



come off in the many processes of staining, and if one has many 

 sections on a slide some of them may become loosened. To avoid 

 this the sections are covered with a delicate layer of collodion, 

 which holds them down to the slide. The early method was to use 

 a soft brush and paint a thin film over the dried sections before they 

 were deparaffined. Now the sections are deparaffined, and then 

 after draining the xylene from the slide, 10-15 seconds, it is put 

 into a bottle containing ^% collodion (§ 395). In a minute or 

 more the collodion displaces the xylene and penetrates the sections 

 and forms a delicate veil over their free surface. No harm is done 

 by leaving the sections in the collodion a considerable time, but a 

 minute or two is sufficient. The slide is removed, allowed to drain 

 for half a minute, and then put into a jar of 67% alcohol (Fig. 222). 

 The alcohol fixes the collodion and removes the ether. As the 67% 

 alcohol does not hurt the tissue it may stay in the jar a day or more 

 if desired, but half an hour suffices. 



Steps in Order for the Paraffin Method. — § 439, 450, 461- 

 471. 



Name 



No. 







Absl. ale. . 



_ Cedar oil . 



Date 



Infilt. _ .. 





Fixer _ _ _ . . .. _ 



Temp. bath. 



.. Imbed, in 



Time of fix __ .. 



Sections cut . . 



-,u's 



Washed in water. 



Temp. room. _ 





67% ale. -82% ale „ 



Stains 





Decalc. g 398.- .67, 82% ale. 



In toto stain . . 







Mtd. iu.. ...... 



Washed in. . ..-_ 



Remarks _. . 



67% ale. .. __82%alc... 



95% ale. and eosin. . ... 











