CH. A'] 



COLLODION METHOD 



3°9 



Fig. 24S. Waste bowl with rack for supporting slides and a small funnel 

 in which the slides stand while draining . This outfit is easily made by any 

 tinsmith. The rack is composed of two brass rods about 4 mm. in diameter. 

 The bent end pieces are sheet lead. The funnel is made of tin, copper or brass. 

 Either copper or brass is preferable to tin. A glass dish like that shown in 

 Figs. /SS, 2ji is better than a bowl, as it can be more readily and thoroughly 

 cleaned. {Cut loaned by II 'in Wood & Co.) 



\ 460. The Castor-Xylene Method of Sectioning. — The preparation of the 

 tissue is the same as described in § 451-456, except that when the collodion is 

 hardened in chloroform it is transferred, not to alcohol, but the block is placed 



Fig. 249. Perforated section lifter. This is easily made by soldering a 

 wire to some very thin sheet brass or copper, and then perforating this with a 

 coarse needle or fine awl. Any roughness must be removed by using a fine 

 oil stone. 



in Castor-Xvlene (I 392). In a few days the collodion gets as transparent as 

 glass and one can see the tissue within with great clearness. It can remain in 

 the castor-xylene indefinitely. 



