316 STAINING AND PERMANENT MOUNTING [ CH. X 



tissue red, muscle, blood and epithelia yellow or yellowish. Arter- 

 ies are excellent for this combination. 



§ 470. Combined Elastic, Mucicarmin and Picro-Fuch- 

 sin Stain. — For this, one should take some object that is known to 

 contain elastic tissue, mucus, white fibrous tissue and muscle. 

 (The non-cartilaginous part of the trachea is excellent.) The organ 

 should have been fixed in mercuric chlorid or Zenker's fluid 

 ($5 § 416, 429) for this preparation. The sections should be made 

 by the paraffin method (§ 439) and no collodion should be used for 

 fastening the sections to the slide (§ 450) for collodion is stained by 

 mucicarmin. 



(1) Stain first in the elastic stain 1 hour. Wash well with 

 95 '/o alcohol and then with water. 



(2) Stain in a shell vial or jar of mucicarmin (§ 389) from 1 

 to 24 hours. Wash well with water, but one must be careful in 

 treating these sections as they have no collodion mantle to protect 

 them. 



(3) Stain 15 to 30 seconds with picro-fuchsin of }(. strength 

 (§ 469). Dehydrate with 95% and if necessary absolute alcohol. 

 Clear in carbol-xylene and mount in acid balsam (§ 387). The 

 elastic tissue will be black or blue black. Mucus will be carmin or 

 rose red, white fibrous tissue will be magenta red, muscle, epithe- 

 lium and blood will be yellow. 



EOSIN METHYLENE BLUE STAINING 



§471. Eosin Methylene Blue. — One of the best objects for 

 this stain is a hemolymph gland. Such a gland is easily and surely 

 found by a beginner if he takes the heart and lungs of a veal. In 

 the fat around the heart and behind the pleura will be found red 

 bodies looking almost like blood clots. Remove carefully, fix in 

 Zenker's fluid or mercuric chlorid, (§§ 416, 429). Section by the 

 paraffin method, make the sections $n and io/< thick. Use collo- 

 dion for ensuring the fixation to the slide (§ 450). Stain the 

 sections 5 minutes in alcoholic eosin (§ 402). Wash off the eosin 

 stain with water. (This is an exception to the generalization in 

 § 461, 2.) 



Stain in methylene blue (§ 417) y 2 to 5 minutes. Rinse well 

 in tap water'. Dehydrate with neutral 95 % alcohol (§ 380) and 



