No. 403.]}  .VOTES ON A SPECIES OF PELOMYXA. 543 
Scattered abundantly through the endosare are spherical 
bodies, having in the living animal a bright appearance with 
a dark contour, and looking much like oil drops. They very 
commonly have a diameter of about 8 microns, though smaller 
ones of all sizes are present. These bodies evidently corre- 
spond to the ** Glanzkórper " originally described by Greef! in 
P. palustris, although in the latter form they may reach a much 
larger size than I have observed in P. carolinensis. These 
*refringent bodies," as Gould? calls them, are insoluble in 
alcohol. They are stained by osmic acid and by iodine (alco- 
holic solution), in nowise differently from the granules of the 
endosarc (Ze., are browned). They cannot, therefore, be of a 
fatty or starchy nature. The contents of the bodies is fluid, as 
may be demonstrated by allowing the Pelomyxas to be gradu- 
ally compressed through the slow evaporation of water from 
under the cover-glass. At a time when the arrangement of 
the coarser and finer granules of the protoplasm is not inter- 
fered with, the globules burst and run together, suggesting 
fat droplets very strongly, but even in this condition osmic 
acid does not blacken them. On the other hand, the globules 
are stained a deep blue with haematoxylin. In sections (fixed 
in Zacharias, stained with Delafield's haematoxylin) it may be 
seen that the endosarc is thickly studded with coarse granules, 
Which stain blue with haematoxylin. The smallest refringent 
bodies, recognizable as such, differ but little in appearance 
from these coarse granules. They are slightly larger, of a 
more rounded shape, and take a deeper stain. From these 
minute globules, all gradations in size may be found up to the 
large ones, 8 microns in diameter. With the increase in size 
the depth of coloration increases — an effect due doubtless to 
the greater diameter. The bodies, when stained with hæma- 
toxylin, present a perfectly homogeneous appearance. From 
the evidence at hand, it would seem to me that the bodies are 
globules of an albuminous nature, consisting of a pellicle, en- 
closing a more fluid substance. Greef (/oc. cit.) inclined to the 
| Arch. f. mikr. Anat., Bd. x, 1874. 
? Notes on the Minute Structure of Pelomyxa palustris, Quart. Journ. Micr. 
Sci., vol. xxxvi, 1894. 
