ON THE INTESTINE OF AMIA CALVA. 
WILLIAM A. HILTON. 
ALTHOUGH much work has been done upon the morphology 
and embryology of Amia, but little attention has been given to 
the microscopic anatomy of the intestine. After an examina- 
tion of sections of Amia’s intestine, and after comparing them 
with what has been done on the subject, it was found that 
there were many interesting but rather difficult problems which 
remained to be solved. In consideration of some of these 
points a more careful investigation was undertaken.’ 
In order to obtain a clear idea of the form of the mucosal 
convolutions, specimens were examined which had been injected 
with and preserved in either formalin or alcohol. For inject- 
ing the capillaries the “Berlin blue injection mass" was used. 
The body was transsected caudad of the vent, and the injection 
made into the dorsal aorta within the haemal arch. In making 
preparations to show cellular structures the usual histological 
fixers and stains were used; such fixers as Flemming's, Her- 
mann's, Zenker's fluids and mercuric chloride solution ; such 
Stains as safranin, iron-haematoxylin and chloral haematoxylin, 
with picro-fuchsin. 
The gross structure of Amia's intestine has been carefully 
described elsewhere (Hopkins, '92), so a simple description is 
all that is necessary to give here. The stomach lies parallel 
to the cephalic part of the intestine and joins it ata very acute 
angle; the pyloric valve marks the junction of the two parts 
and appears on the exterior as a very deep constriction. In- 
ternally, the pyloric valve projects as a tube a short distance 
into the cavity. The first part of the intestine is quite long 
and extends in a parallel line for about two-thirds the length of 
l The work was done in the histological laboratory of Cornell University. 
I wish especially to thank Professors Wilder, Gage, and Kingsbury for their 
valuable suggestions relating to the investigation. 
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