328 PROFESSOR LISTER ON THE GERM THEORY 
The filaments closely resembled those seen in this glass a year and a quarter 
before, except that they were invariably very short, and the corpuscles, while - 
sometimes in groups more or less resembling the original torula, were often of 
a more elongated form and strongly nucleated. During the first five days after 
the inoculation there was no distinct appearance to the naked eye of any 
growth taking place in the new glass of PastTeur’s solution. At the end of that 
time, however, thinking that a speck of delicate scum, which existed from the 
first, appeared slightly increased, I examined a portion microscopically, and 
found it to consist entirely of cells which appeared of new formation, some of 
them presenting transitional forms between the elongated bodies common in the 
test-tube and the constituents of the oval torula. The growth afterwards con- 
tinued, both as a very delicate scum, and as a fine white deposit; but its rate was 
extremely slow, and the product for the most part on a much smaller scale than 
the original torula, and more resembling the elements found in the test-tube. 
Very different was the behaviour of the organism in unaltered urine. Two 
days after the inoculation of the PAsTEur’s solution, I introduced half a minim 
of the liquid from the test tube into a “heated” and covered glass, containing 
unboiled urine from a flask which had been charged on the first of March, but, 
though it had furnished the material for many successive experiments, retained 
its original characters unimpaired.* For two days there was no appearance of 
growth ; but on the third day a small patch of scum, which had been the im- 
mediate result of the imoculation, was considerably increased in size, and had 
acquired a much coarser character, and several small detached specks of similar 
aspect were floating on the surface. The side of the glass was also sprinkled 
with minute particles like grains of white sand, often disposed in vertical 
streaks, while other similar granules were deposited at the bottom, the liquid 
retaining its brilliant clearness. In short, the naked eye appearances were an 
almost exact reproduction of those which resulted from the introduction of the 
rain drops into the original urine nearly two years before, and on applying the 
microscope to a portion of the scum taken up with “heated” pipette, I was 
delighted to find it composed exclusively of the Torula Ovalis in all its original 
beauty, the constituent cells pullulating freely, as shown at 2, Plate XXIII., which 
represents, for convenience of sketching, a small specimen of the groups, which 
were commonly much larger, like those of the yeast plant when in full activity. — 
In some fields the cells were peculiarly large, as at m, and here and there, as at 
Zand n, a cell was somewhat longer than usual, just as occurs in Torula Cere- 
visie ; but there was no appearance of filamentous growth. It was a torula 
pure and unmixed ; yet its identity with the Zorula Ovalis and its distinction 
from the yeast-plant were declared not only by the form and aspect of the cells, — 
* The method by which this flask was prepared, and the mode of decanting into the experimental 
glasses, will be described in a later part of this paper. : 

