
OF PUTREFACTION AND OTHER FERMENTATIVE CHANGES. 333 
which the pocket magnifier had revealed. Under a low power they presented 
appearances such as are shown at a, b, and c, Plate XXV., a being a purely 
filamentous growth, ¢ a granular group, and 0 one exhibiting both characters 
in combination. Under the high power the granular parts were found to be 
composed either of groups of free pullulating cells of oval form, generally 
disposed in pairs, as shown at ¢, Plate XX V., or of plants of a most imperfect 
description, consisting of cells of a similar character to the free ones, or slightly 
more elongated, connected end to end, and often producing conidial buds, as in 
the specimen figured at d in the same plate. 
On the following day the difference between the two glasses was still more 
marked. The filamentous plants in the Pasteur’s solution had considerably 
increased, but those in the urine had almost all fallen to the bottom, their 
places being taken by abundant specks and streaks of granular aspect, and even 
the few plants that still remained adhering had lost their purely filamentous 
character and had become granular. There were also little patches of scum 
upon the urine, whereas the surface of the PasrEur’s solution presented only some 
floating filamentous plants. I removed a portion of the scum with “heated” 
pipette, and submitted it to the microscope, and found it to consist exclusively of 
free oval cells, like those seen in the granular specks the day before, as shown in 
outline at 7 In the course of the next twenty-four hours all appearance of fila- 
mentous growth disappeared from the urine; but while the liquid, which was 
now for the first time observed to have a slightly offensive smell, had become 
unsuited for that mode of development of the organism, it had stimulated the 
corpuscular form in a most remarkable manner, the scum having increased with 
amazing rapidity. Thus, between 8 p.m. on the 24th and 5.30 a.m. on the 25th, 
it grew from a loose patch, about half an inch in diameter, to a dense film that 
_ covered almost the entire surface of the liquid in the urine glass, and eight hours 
later, the cell growth had been so great that the scum had become pushed up 
upon the glass to about a quarter of an inch above the level of the liquid, while 
the urine was rendered cloudy by the subsidence of detached cells. In the 
course of the afternoon the liquid had become turbid throughout, and the air in 
the glass shade was still more decidedly offensive; yet, under the microscope, 
the only organism discoverable was that represented by the pairs of cells before 
described, so that we have here another clear example of fermentative change 
of putrefactive character induced in urine by other agency than bacteria. 
Samples of the cells are given in g, Plate XXV., where they are seen to 
resemble those of d and e in having vacuoles, but no nuclei, merely, in some 
cases, inconspicuous granules. In g is also given in outline a portion of the 
scum, showing how densely packed the constituent cells were, corresponding 
with the remarkable naked eye appearance, which was that of a dense white 
layer, like a film of paraffin. 
VOL, XXVII. PART III. 4s 
