334 PROFESSOR LISTER ON THE GERM THEORY 
On the same day (August 25), I introduced a small portion of this scum 
into a second glass of urine, prepared along with the former one fifteen days 
previously, but as yet retaining its brilliant clearness, and in other respects also 
unaltered, the transference being effected by a touch with the tip of a slender 
glass rod previously “heated.” The results of this inoculation differed from 
those seen in the former glass of urine in this, that no filamentous plants at 
all now made their appearance, while, on the other hand, the corpuscular mode 
of development proceeded with great rapidity. Thus, eight hours after the 
inoculation, the side of the glass already presented streaks having a granular 
aspect under a pocket-lens, and a portion of scum which had remained at the 
surface had increased to four times its original extent, presenting the same 
dense white character as in the former glass, like a film of wax or paraffin. I 
examined a portion of the scum microscopically, and found it to consist in the 
main of cells, free or in pairs, formed by pullulation, as shown (for the most 
part in outline) in the sketch given at 2, Plate XXV. But there was besides 
frequently seen an appearance of somewhat longer sprouts, like an abortive 
attempt at the formation of filamentous plants, of which also specimens are 
given in the sketch 7. Twelve hours later the inside of the glass looked as if 
sprinkled over with coarse white sand, while the scum had grown so rapidly as 
to be more than eight times as large as when last observed. A portion of scum 
is represented in outline at 4, where it is seen that there is no longer any — 
appearance of long sprouts, the filamentous tendency having entirely disappeared, 
while the constituent cells are of smaller dimensions than before. Another 
point of much interest was the fact, that now, within twenty-four hours of the 
inoculation, the liquid, which, when inoculated, had the odour of perfectly fresh 
urine, was already markedly offensive; and after the lapse of twenty-four hours, 
while the scum had almost covered the surface of the liquid, the rank smell was 
strong. Now it may be remembered that in the former glass no offensive smell 
was observed during the first three days, though the filamentous growth had 
proceeded luxuriantly, and that it was only after four days, when the filamentous 
form had given place to the corpuscular and the scum had made its appear- 
ance, that the rank odour was perceived. Hence we are led to infer that the 
same organism may differ in its effects as a fermentative agent according to its 
habit, the toruloid form in the present instance being a much more energetic 
ferment than the filamentous. I had the opportunity of verifying this observa- 
tion seventeen days later, when another glass of urine being inoculated with 
the same scum, there was again a rank smell in twenty-four hours. 
But to return to the glass under consideration. On the 27th August, two 
days after inoculation, it stank as strongly as the first glass; and now on exam- 
ining a portion of the scum with the microscope, I was surprised to find a very 
remarkable change in its constituent cells, which, instead of being oval bodies 

