338 PROFESSOR LISTER ON THE GERM THEORY 
cells. Early next morning I found that the cells generally were sprouting; but 
it happened that those which I had drawn had shifted their position slightly, 
so that I could not distinguish them in their now altered shape from others in 
their vicinity, but I selected two groups for further observation, represented at 
b, and ¢,, sketched at 1.30 and 1.35 a.m. respectively. It will be observed that, 
while this early stage of germination has changed them from the spherical to 
an oval form, they still retain their nucleated character. Five hours later, 
growth had advanced in both groups so as to give the appearance represented 
at 6, andc,. In both groups the nuclei have almost disappeared, while the 
sprouts have much increased; and in c,, while the highest of the three cells 
has produced a short filament, the lowest has formed two oval vacuoled cells, 
and the other, after growing an oval cell, has gone on to the development of a 
short filament. 
After four more hours had passed, I was rejoiced to find the experiment 
crowned with complete success. The longer sprouts of ¢, had become extended 
to threads of considerable length, as represented in c,; while the progeny of the 
other original cell was in the form of pairs of oval vacuoled bodies destitute of 
nuclei, exactly resembling the constituents of the first scum, or of the granular 
deposits which accompanied the woolly tufts on the first urine-glass. And just 
as in that glass, at an early period, some plants exhibited the filamentous, and 
others the corpuscular form of growth, so was it with the offspring of the three 
spherical cells whose development we have followed. 
Such was the effect of uncontaminated urine upon this organism. After- 
wards, however, as the liquid gradually became vitiated under its fermenting 
influence, the filamentous form of growth which first appeared began to give 
place again to the corpuscular, a change which the “glass garden” afforded 
opportunity of watching with perfect precision. c, shows the lower of the 
two filaments of ¢, at 5.50 P.M. on the same day, represented on a smaller 
scale. It will be observed, that, while the filament has increased considerably 
in length, it exhibits a tendency to break up into segments, and here and there 
along its course it has produced oval corpuscles. And a further progress of 
the same alteration of habit is exhibited in c,, where the same filament is again 
sketched on the same scale after the lapse of ten hours more, viz., at 3.50 A.M. 
on the 18th September. The filament has only increased very slightly in length, 
but the terminal portion has broken up into segments, and assumed a zig-zag 
form in consequence, while a multitude of corpuscles have been produced in 
the course of the filament, partly by budding of the segments of the thread, 
and partly by the pullulation of the corpuscles themselves, many of which 
are already of the spherical form. And the spherical cells, when examined 
with a high power, were found to be nucleated like those of the last scum. 
Here and there a plant was found in which, in consequence, I presume, of 


