498 PROCEEDINGS OF SCIENTIFIC SOCIETIES. 
Dr. A. 8 
Limulus Polyphemus." 'The eggs on which the following observations 
were made were kindly sent me from New Jersey, by Rev. Samuel Lock- 
od, who has given an account of the mode of spawning, and other 
habits, in the AMERICAN NATURALIST. They were laid on the 16th of 
May, but it was not until June 3d that I was able to study them. The 
eggs measure .07 of an inch in diameter, and are green. In the ovary 
they are of various hues of pink and green just previous to being laid, 
the smaller ones being, as usual, white. The yolk is dense, homogeneous, 
and the yolk granules, or cells, are very small, and only in certain speci- 
mens, owing to the thickness and opacity of the egg-shell, could they 
Not only in the eggs already laid, but in unfertilized ones taken from 
the ovary the yolk had shrunken slightly, leaving a clear space be- 
Embryo of Limulus. 
tween it and the shell. Only one or two eggs were observed in- process 
of segmentation. In one the yolk was subdivided into three masses of 
unequal size. In another the process of subdivision had become nearly 
completed 
, 
of three minute, flattened, rounded tubercles, the two anterior place 
* t H 
of Savigny), being much smaller than the others. The mouth opening 
is situated just behind them. In a succeeding stage (Fig. 95, ar, areola; 
am, blastoderm skin; ch, chorion) the embryo forms an oval area, SUT- 
rounded by a paler colored areola, which is raised into a slight ridge- 
This areola is destined to be the edge of the body, or line between the 
ventral and dorsal sides of the animal. There are six pairs of appen- 
dages, forming elongated tubercles, increasing in size from the head 
