115 
amber-coloured masses of gonidia involved in mucus already described, 
The eee are simple or branched, variable in length, extending 
to 25 p the average being about re s 3 v the gonidia are constant 
in form an Po measuring 9-1 *6-4 p, and, seen by transmitted 
light are almost colourless. SWR in water these gonidia germinate 
within 12 hours, emitting a germ-tube from near one end, which soon 
produces short lateral branches at right angles; the whole perishes 
ks nar Me three days, and in several cultivations the development 
ed beyond the condition described above. When sown in a 
sterilized Mieatioh of raisins and gelatine, the gonidia germinated as 
m s in its growth, 
ww 
"i e. 
small ace colourless, secondary gonidia measuring 4 x 2 
cut off from tips of most of the branches. 
These ese gonidia, after becoming gus reproduce them- 
selves to an enormous extent by budding, but were never seen 
germ-tubes. In flask cultures in a sterilized, Wi solution of plums the 
gonidia within 10 days give origin to a copious weft of mycelium which 
oduces microgonidia below the surface of the Soan these reproduce 
themselves so rapidly by budding that the originally clear solutio 
becomes turbid, and this continues until the nutritive material is ex- 
hausted, roman the mass of yeast-like cells sink to the bottom like a 
precipitate. - No further development could be obtained from the cells 
produced by budding although sown in varions nutritive solutions, 
including the liquid from crushed living vanilla leaves obtained from the 
Royal ne Kew. 
A quantity of leaf-mould mixed with water was thor PEE sterilized 
by intermittent boiling in a flask until the water had evaporated, leaving 
the soil moist, into this was ned two drops of the Beeson of plums 
containing the budding condition of the secondary gonidia, the flask was 
closed with sterilized cotton wool,and kept at a temperature of about 70°F. 
for six days, when a quantity o of — recently boiled and proved by mi- 
croscopic examination to be pure was added, and e whole thoroughly 
shaken up; on examination the ieee was foun 
budding forms of the secondary gonidia in all << of Com a nig sen 
prolonged examination failed to show MT. eros x dev sone ment. 
Some in si 
'z 
phase of development was observ 
The above a quie that when the Hainsea gonidia are 
washed off the leaves by rain and carried to the ground, they germinate 
and produce sc atte cane of cells by budding, but what these cells 
eventually ae rigin to is unknown. Living vanilla leaves were 
infected with the Hainsea gonidia, but always without result, although 
tried in a se of ways both on the upper and under suface of the 
leaf, and by being placed in mid parts, from which it may be con- 
cluded that the y amem M reproduce itself directly on the living 
vanilla plant. 
the time the Hainsea is -fully developed, owing to the ravages of 
its mycelium, the leaf, has become yellow and is evidently dying, and at 
