118 
continues to elongate and becomes Netus man forming the nec 
the st e of 
complete oe a. a cavity into which the ille or 
thecium, serving as a nucleus round which the wall forms, and eventually 
disappearing when the asci develop, these latter soon completely filling 
the ready-made cavity. The origin of the neck-canal was not deter- 
mined, but it certainly takes place at a Inter period than the basal 
cavity. The asci when mature are — and attenuated 
2-14u. 
below into a slender Soner n: e measure 
e spores are eight i in ascus, Bine sub-cylindrical 
with rounded ah is slighty sid, solos ess, and triseptate at ma- 
turity, and m x54. Par raphyses scanty, linear, equal, 
pie aap: 50-100 d 
was ved, the mycelium dying for want of nourishment. In a nutri- 
tive solution the spores germinate as described but growth is much 
more vigorous, and in about three weeks minute pustules were 
formed by panicles of short, erect hyphe, from the t tips of which gonidia 
were cut off resembling in n form and size those produced by the Hainsea 
orm on living leaves. In the cultures, the stroma was redline or 
entirely absent, but the gonidia Sibel Se in a decoction of plums in 
4 manner exactly similar to those obtained from the leaves, and also 
repeated themselves by budding, ind there seems no room for doubt but 
that the gonidial form of reproduction produced by the germinating ge 
of the Calospora agree with Hainsea found on living vanilla es. 
The accuracy of the above statement was verified by the appearance of 
the Hainsea on living vanilla leaves obtained from ~ 7 al Gardens 
about three weeks after being infected with Calospo a spores. The 
leaf that produced the Hainsea was placed under a bell jar with its base 
in water; another infected leaf suspended from the top of the same bell- 
jar yie elded no result; from this it appears that the presence of an excess 
of moisture in the leaf favours the development of the fungus. The 
ascospores were placed on the uninjured upper surface of the leaves. 
The germ tubes of the Calospora enter the tissues of the leaf through 
the stomata, and the leaf is the only portion of the plant through which 
X 
production of the Hainsea from the Calospora closes the life-history of 
the fungus under consideration, although the proof from ers 
cultures is not a complete pu senda a instance, the Hain 
could not be made to give origin to the Cytispora form, nevert sikala 
the cycle has been completely road s as g aote the perithecia of the 
Calospora has been shown to originate from the stroma of the Cyti- 
spora, and the Hainsea ie been produced from the germinating spores 
of Calospora. 
