14. 



■or»owr the bsolsity of the bicirbcntt* (p.H. glwan in last two columns tnbls 5a) 

 ia sufficient to account for the slight iepression in regens rotten caused by thsa 

 «t compared to the control, it »»y r.nttslj be concluded th«t the ieprsssion in 

 regeneration caused by basis is das to t'ne hydroxy! ions. fhla conclusion 

 ssess ths xaom Justifiable in visw of ths fnot that the concentration.* of salts 

 used ia ths la ltl experiments are three to five times ns great as the concen- 

 tration* of ths hylroxlds used. 



C a rb on Bloxlls Pro! notion e n J gogene ra tion in Brnoe e 



As basic me! la .are generally kno»n to increase oxygen aetsbolism, 

 it was thought desirable to try a few experiments to see whethar the decrease 

 in regeneration eoul.t in Any way be correlated *t incre-ia-" in taffbon dioxide 



production. 7hs methods employed have already been described. 



Table 7, experiment 4, gives the CO^ production in KOH of six 

 sets of four tadpoles eaoh. Table givso the regeneration of ths esse tad- 

 poleo. Those i~i tie higher concentrations (30, 35 aad 40 c.c. KOH per 400 e.c.) 

 died without regeneration. Tig. 9 (drawn froa table 7) repreeent* graphically 

 the Increase in C0 3 production with increase! concentration of base and gives 

 a curwe almost identical with the ourvs shown in Fig. 10, which was drawn froa 

 the data of Loeb and ITastnay (1913 b) for ths 1 nereis* of oxygen consumption in 

 increasing concentrations of bases. 



Fig. 11 gift's the ourwee of growth for the three sets of tadpolee 

 which survived. It shoro clearly the decrease both in the mte of regeneration 

 And the total amount regenerated in increasing concentrations. 



Fig. 12 (drnwn froa th-* data of tables 7 and 8) compare* the 

 effect of the varying concentrations of K3QRI upon the rate of regeneration taken 

 ss time necessary to regenerate 20, 30 tad 35$ of ths »'rount removed. The 

 dotted line represents the CO^ per gram per day produced by the s-iae tadpoles. 



