8. 



supported the Ufa of tadpolsa of ftufo lantlyiflos ua. Le Conte through metamor- 

 phosis. These results are in accord with tits finding of Bullot (1914) an* 

 Powers (1919 b) who attributes the toxic effect of ordinary distilled tator 

 to tr-ieea of colloidal coppar taken ftp from the condenser. Powers had shewn 

 that a goldfish nay live fifty-one 1*ys in '*iter redistilled in glass byt 

 succumbs quickly if ths minutest trace* of colloidal copper be aided to tha natar. 



The water for all of the exporia&ente was onionaed in a six-foot 

 Jana glass eondanaar attache! to tha second tank of the watar-b oiling apparatus 

 •stationed above. It was than aerated for 24 hours by a atrosa of air pre* 



viously paaaad through sulphuric acid and calcium hydroxid rash bottles. This 

 dlatllled water »** neutral to Rosalie acid and neutral rai. An enalysia 



of the water kJr. J .ly made by tnm water analyst of the State ffater Survey was 

 as follows: 



Physical examination 



Turbidity 



Color 5 



Odor V 



Residue on evaporation 



Total eolide 33 to 42 part* p&r million 



Alkalinity as calcium carbomte 



Methyl orange to parts per million 



Chlorides 



At sodium chloride to parts per million 



Asmonia nitrogen 4 to 7 parts ■pnr million 



Albuninoid ammonia 0*060 to 0.102 parta per million 



A oonwon shiner (Notropus blennius) lived in t-tia water (changed weekly) from 



Octobar IS, 1917 to Pebniary 17, 1918, or a total of one hundred twenty-two 



iaya. As, luring this tim;*, the fish deoreaaal notably in ai»t, ita death 



