262 
By Watt’s method of рые just detailed it is only possible to 
isolate a portion of the antiarin contained in the fresh sap, owing to the 
о olour] a water 
bath till the antiarin crystallizes out. Then freeing the crystalline mass 
from pri sulphate of lime which may be present by dissolving it in 
One Я ed, 
10-85 grains of — or 2-482 per c The dried Ipoh poison, of 
which the sap contains 29 per cent., therefor ore has 8-561 per cent. of 
antiarin in it, агли by the above | proce 
With the imperfect laboratory а ROR at my disposal these results 
eannot be considered as more than estimates of the total amount of 
poison present; and unfortunately I have no analysis of Upas with 
€ to compare them. 
e result of weighing coated darts and washing off the poison and 
чбс the amount of Ipoh on them may be taken to be rather less 
жете grain, апа by nee estimate above given this weight of the dried 
ould only contain · i of a grain of the alkaloid ; which, how- 
ever ci suflici ent to produce a fatal result when introduced into the 
circulation of an animal weighing as much as 
n the afternoon of t ay on which the чы was сакой I tried 
the following ticle with it on two fowls 
Experiment 1. 
June 9, 1891.—Three minims of the fresh sap was injected hypo- 
dermically with a syringe, without drawing blood, into the leg of a fowl. 
- In three minutes the leg was affected and tho bird was rather lame, 
but for half-an-hour remained otherwise quite well, eating freely and 
walking about. It then became rather dull and sluggish, and voided at 
very frequent intervals 
Twelve hours after the injection it was apparently not much the 
worse for the operation, but a large ~~ patch had formed where the 
injection had been made, covered with loose skin and surrounded. by 
nae inflammation, and radiating Б it were several enlarged blood 
ST wanty hours after it was very lethargic. 
Forty-two hours after the inflammation was oe but the = 
was still very dull, though I have no doubt of its ultimate recovery, b 
as I had to leave the place, could not continue prede on it. 
Experiment 2. 
June 9, 1891.—А poisoned dart point, which had been prepared 
ore me from the same sap, was stuck into the leg of a fowl and slight 
lameness and діагћоа came ios but no other symptoms. 
A second arrow was stuc re other leg after half an hour had 
| y ordiri but — уч fet tol owed 
! tehes surrounded the wounds, and 
there was чаба чийе ke daa adjacent parts and considerable 
