213 



I should mention that the colours of I5, IIj and IIIj were absolutely 

 equal at the beginning of the experiment; this was also the case in 

 L— I,,, II3, II4, III3 and III4 as well as in 1,2—1,5, II^, lie III5 and 

 Illg. The experiments Ig etc., II3 etc. and III3 etc, contained other 

 ,, enzyme" and ,, emulsion" than the preceding ones. 



In the first place we see from the table, that the ,, blind" experi- 

 mental series II and III lose indeed more or less their red colour. 

 Consequently, acids are set free in these. In II one might ascribe 

 this to hydrolysis due to the lipase, for this mixture must have also 

 contained some (few) oildrops. But in III no lipase can have been 

 present at all; nevertheless the fat is hydrolyzed — probably by means 

 of the alkali — (or the red colour diminishes by the entrance of CO^ 

 from the air). 



The experiments of series I, liowever, prove to lose their red colour 

 sooner than those of II and III. One might be inclined to explain 

 this by the combined influences, which were acting separately in II 

 and III. But that would not be exact , as is shown in the second and 

 in the last group of experiments. Still another factor must have been 

 acting. That must be the hydrolysis of the fat by the lipase. But 

 this lipase does not seem to be very active here; although it is diffi- 

 cult to give a decision. 



Table 14. llie ntnuher of oildrops and of globules, ivhich can he 

 stained (hroum) by ƒ, present in choanocytes in comparison to that 

 in amoebocytes, in green and colourless Spongillidae taken from 

 water of the lake, from water of the canal and from that of the 

 conduit; microscopically examined by means of ravel preparations 

 (p. 12 — 15) of sponge tissue. 



I examined green Spongillae (s.) and Ephydatiae (e.) from light and 

 colourless ones from darkness. The (average) number of oildrops 

 (oildr.) and of globules which can be stained by I (glob. I) was always 

 stated in the same volume of amoebocytes (sub 1) and of choanocytes 

 (sub 2). As for the meaning of I — XII, see pag. 14. In the column 

 ,, remarks" is mentioned: the species of the sponge; the month of 

 the examination; whether/the sponges, after their capture from the 

 lake or the canal, had been in water from the conduit (aq.) before 

 they were examined, and for how many time; and whether sponge- 

 reduction had occurred. 



All experiments are mentioned. As for the discussion, see pag. 90 — 93. 



