784 Methods of Microscopical Research [October, 



The shellac is prepared and used in the following manner : 

 One part of bleached shellac 1 mixed with ten parts absolute 

 alcohol, and filtered. The object glass is first warmed to about 

 50 C., 2 and then a thin film of the shellac is laid on by a 

 glass rod drawn once over its surface. Before using, the slide is 

 again warmed, and the shellac surface washed with oil of cloves 

 for the purpose of softening it. The wash is made with a small 

 brush drawn back and forth until the entire surface has been 

 moderately but evenly wet with the oil. Sections are now cut 

 and arranged for the first cover; this done, the slide is warmed 

 over a spirit lamp so that the paraffine adhering to the sections 

 melts and flows together, forming an even layer which cools al- 

 most instantly, and thus secures the position of the sections 

 while those of the second cover are prepared. The sections for 

 the last cover having been completed, the slide is warmed for ten 

 minutes on a water bath, in order that the sections may sink into 

 the shellac and become fixed, and the clove oil evaporate. After 

 allowing the slide to cool the process is concluded by washing 

 away the paraffine with turpentine, and inclosing in balsam dis- 

 solved in chloroform. 3 





spared for artists as 



a '//.«-,, 



, 



It is perfectly trai 



,-.;>:!- 





















all label to 





. 



es "for the number of the 









marks the shellac 



side 























:d throughout tb 















my attention has been call* 



:d to the following 1 













d by Dr. Gaule (Arch, v. 







Phys. Abthlg 



- P- '56,): 













: 





id placed on the slide in the order and position in ■ 



















i 



e then smoothed 









;;;' 



iho!, until 



all wrinkl( 



iS are R 



moved and every part is in 



close contact with u- 





|. Slide all 



lowed to st: 



m.l sevi 



sral hours (or over night) n 



ntil the alcohol has 





pic 



tely evaporated, and 







firmly to the glass. 





















.. Tte par 











, !S 



















]' 'Vn'/'.^'r 'vi^in equal 

































Dr. Gaule 













gla* 







' -« ■ ' 





igle slide may often 

















Dr. 































laii. 1 



11 the case of hod 



,'s, not liable to get 1 







; during tl 



le placing, 



I prefei 



■ the shellac method. 







