is needed on paralytic shellfish poisoning; 10) depuration programs should be 

 expanded in the natural environment and onshore; 11) more extensive use should 

 be made of aquaculture; and 12) increased effort is needed in mission-oriented 

 biological and public-health-related research. - J.L.M. 



1370 



Natural Resources Institute. 1970. 



Assateague ecological studies. Final Report. I. Environmental information. 

 Univ. Md., Nat. Res. Inst. 



Unable to locate. Search terminated. - M.W.S. 



1371 



Natural Resources Institute. 1970. 



Assateague ecological studies. Final Report. III. Assateague study, 

 suggestions for land use and park management. Univ. Md . , Nat. Res. Inst. 



Unable to locate. Search terminated. - M.W.S. 



1372 



Navez, A. E. , J. D. Crawford, D. Benedict, and A. B. DuBois. 1941. 



On metabolism of the heart of Venus mevoenavia. Biol. Bull. 81(2): 289-290. 



Excised heart of hard clam will keep its characteristic contractions for a 

 long period in a small quantity of "aerated sea water. This is so when the 

 heart is whole, cut into a few pieces, or chopped into many small pieces. 

 In any form, heart tissue will respire at a uniform rate for long periods. 

 "Cut" heart exceeds whole heart in 02 consumption by about 10%; "chopped" 

 heart is lower by about 10%; "minced" heart about 50% lower; but all seem 

 to fix 02 with an RQ around 1.0. This applies to heart unwashed with sea 

 water. Washing with sea water lowers the rate of the reaction, but 

 addition of the washings usually restores the rate to normal. "Minced" 

 pulp can be centrifuged into 2 components: supernatant fluid, which 

 respires at about 30-35% of the rate of the original "minced" pulp; and 

 granular part 65-70%. Acetonic extracts and residues reunited in water are 

 inactive or inactivated. The cytochrome-cytochrome oxidase-dehydrogenase 

 system was used as a working hypothesis of functioning of the respiratory 

 system, because cytochrome C and succinic dehydrogenase were present in 

 Mercenaria heart. Addition of p-phenylenediamine determines a large 

 increase in Q02 depending on concentration, which persists for long 

 periods. Poisoning of heart by KCN determines an inhibition of 40% at 

 most, whether p-phenylenediamine is present or absent. Addition of 

 succinate alone raises Q02 by 10%; succinate+methylene blue increases it 

 to 150% max, but methylene blue alone does the same. Inhibition of this 

 reaction by KCN is 30-40%. Na fluoride, azide, iodoacetate and selenite 

 had small or no effects. Ethyl urethane alone enhanced. A strong 

 lumiflavin reaction points to presence of a f lavoprotein. A weak 



glutathione reaction is given also. It was concluded that the observations 

 did not fit the working hypothesis, and other unreported experiments 

 confirmed the rejection. Additional work was planned. - J.L.M. 



1373 



Neff, Jerry M. 1971. 



Electron microscope histochemistry and biochemical characterization of 

 alkaline phosphatase in the mantle of the clam Mercenaria meraenaria . 

 Am. Zool. 11: 661-662 (abstract 224). 



Alkaline phosphatase activity was associated with the microvilli of the 

 columnar epithelial cells of the outer surface of the outer mantle fold 

 and with the microvilli of the cells of the general outer surface of the 

 mantle above the pallial line. It was also associated with the apical 

 zonulae adhaerens of these cells. No activity could be detected in the 

 cells lining the periostracal groove. Calcium but not magnesium enhanced 

 alkaline phosphatase activity. In the presence of lOmM calcium, magnesium 

 was slightly inhibitory. - J.L.M. 



385 



