392 TRANSACTIONS LIVERPOOL BIOLOGICAL SOCIETY. 



rapidly emptied of all its water by a wide siphon tube, and 

 care was taken to remove all debris and dejecta from the 

 animal. This precaution is necessary to ensure the health 

 of the animals, and it is remarkable for how long periods 

 small amounts are obtained in absence of all food, 

 apparently due to some continued intestinal excretion. The 

 •carboys were then completely filled with fresh sea-water, 

 and closed from the air by tightly fitting cork bungs. In 

 the latter part of each experiment, light was excluded by 

 means of a barrel inverted over each carboy. In the 

 earlier days there was no vegetation, and the results were 

 only interfered with partially for a few days before the 

 use of the barrels began. 



The details of the experiments are given in the four 

 following tables : — 



Experiment I. 



Lobster.— Weight 203-210 grams. Volume of sea- 

 water in carboy = 4556 litres daily. Duration of experi- 

 ment, August lOth-September 13th, 1912 = 34 days. 



Oxygen appearing 



Oxygen used up. in Carbon-dioxide. 



Date. Time , * , , * v 



Interval. Milligrams Total Milligrams Total 



h. m. per litre. mg. per litre. mg. 



August 10 6 10 2-38 108 3-2 146 



„ 10-11 17 50 5-81 265 2-6 118 



„ 11-12 26 45 5-71 260 6-7 305 



„ 12-13 18 10 2-84 129 2-2 100 



„ 13-14 24 311 142 3-8 173 



„ 14-15 23 15 304 139 3-5 159 



„ 15-16 23 30 2-89 132 2-9 132 



„ 16-17 23 50 3-74 170 4-2 191 



„ 17-18 23 35 3-45 157 4-2 191 



„ 18-19 23 35 3-38 154 3-2 146 



„ 19-20 23 55 3-25 148 2-9 132 



„ 20-21 23 30 2-32 106 3-5 159 



„ 21-22 23 20 2-75 125 4-2 191 



„ 22-23 23 55 3-33 152 2-2 100 



The experiment was here interrupted, to attempt 

 feeding, from August 23rd to 25th. The animal did not 



