SEA-FISHERIES LABORATORY. 397 



The carboy was kept in darkness from this onward, 

 (see note in previous experiment). 



Oxygen appearing 

 Oxj^gen used up. in Carbon- dioxide. 



* . * . 



Date. Time 



Interval. Milligrams Total m.g. Milligrams Total 



h. m. per litre, in Carboy, per litre. m.g. 



September 1-2 24 00 2-88 135 4-5 211 



2-3 24 00 3-11 146 4-6 215 



3-4 23 40 2-81 132 4-8 225 



4-5 23 55 2-58 121 40 187 



5-6 23 30 0-96 45 10 47 



The last day given above was carried on partially in 

 daylight, which accounts, as before, for the drop. The 

 animal was offered food here, but refused it. Weight, 

 240 grams, as before. 













Oxygen appearing 









Oxygen 



used up. 



in Carbon-dioxide. 



Date 





Time 

 Interval. 





i 



X 







Milligrams 



Total m.g. 



Milligrams 



Total 







h. m. 



per litre. 



in Carboy. 



per litre. 



m.g. 



September 



7-8 



. 23 55 



4-21 



197 



3-2 



150 



f . 



8-9 



. 23 20 



3-19 



150 



2-6 



122 





9-10 



. 23 50 



2-42 



113 



1-9 



89 





10-11 .. 



. 23 45 



2-79 



131 



1-6 



75 





11-12 .. 



. 23 50 



2-63 



124 



2-9 



136 



,, 



12-13 .. 



. 23 55 



2-71 



127 



3-2 



150 



Experiment stopped, weight of animal, 248 grams. 

 The animal was preserved in absolute alcohol for analysis 

 of tissues. The total amount of oxygen used up by this 

 lobster amounts to 4'33 grams, and the oxygen appearing 

 in carbon-dioxide is 461 grams, yielding a respiratory 

 quotient of 0'93. Making allowances for interruptions, 

 &c, the time-interval totals 27 days, and therefore the 

 daily oxygen consumption averages 0*16 gram, which 

 would oxidise 0*15 gram of carbohydrate or 0'18 of protein. 

 This experiment throughout corresponds very closely with 

 the preceding one, the rate being slightly lower, for the 

 animal was somewhal heavier. It is to be noted that this 

 animal took no food whatever throughout the experiment. 



