412 TRANSACTIONS LIVERPOOL BIOLOGICAL SOCIETY. 



One set of four lobsters were immediately killed by 

 immersion in absolute alcohol, and preserved in this fluid 

 for some months to furnish control observations of dry 

 body-weight, proteins, carbohydrate, fat and ash at the 

 end of the experiments. 



The second batch of four lobsters were placed in the 

 four carboys, which were freshly filled daily with sea- 

 water, as described in the preceding paper, with the 

 exception that the water was not filtered through silk, so 

 that the animals could have had the whole of any possible 

 nutritive material contained in the daily 40-50 litres of 

 sea-water that formed their supply. The animals had no 

 food whatever during the experiment. Daily determina- 

 tions of oxygen consumed and carbon-dioxide formed 

 were carried out over the period from April 9th till 

 May 11th, 1913, that is to say, a period of 32 days, and 

 these showed throughout a somewhat slower metabolism 

 than in the summer months, as recorded in the previous 

 paper, the daily intake of oxygen averaging 120 milli- 

 grams per lobster, instead of the 160 milligrams of the 

 summer months. Apart from this slower rate, the 

 results are exactly similar to those detailed before, and 

 so the tables of the experiments need not now be 

 reproduced. 



At the end of the thirty-two-day period, the animals 

 were weighed, the weights showing practically no change 

 as a result of the 32 days' fast. Thus the weights before 

 were 218'3, 292*4, 258'0, and 265'6 grams, and afterwards 

 221*0, 291*5, 2554, and 265*3 grams respectively— the 

 aggregate weight at the start was thus 1034*3 grams, and 

 at the end 10332 grams. After weighing, these four 

 lobsters were placed in absolute alcohol, and preserved 

 therein like the controls. 



The third series of four lobsters were weighed and 

 placed in the four carboys, soon after the conclusion of 



