220 TRANSACTIONS LIVEEPOOL BIOLOGICAL SOCIETY. 



Weber and Taute (1906) isolated from fresh frogs, 

 from aquarium mud, and from moss 36 strains of 

 acid-fast bacilli, all of which were pathogenic for cold- 

 blooded animals and harmless for warm-blooded animals. 



A large amount of experimental work has been 

 done attempting to prove, firstly, that the organism 

 affecting cold-blooded animals is a changed tubercle 

 bacillus; and secondly, that it is possible, experimentally, 

 by passage through animals, to cause the tubercle 

 bacillus of the human or bovine type to undergo a 

 metamorphosis into the cold-blooded or piscine type. 



Terre (1902), without success, injected and fed 

 fish and frogs with cultures of human and avian 

 tuberculosis. 



Dieudonne and Herzog (1903) showed that frogs 

 inoculated with tubercle bacilli do not die of tuberculosis, 

 but may contain tubercle bacilli in their organs as long 

 as 60 days after the experimental infection. They 

 also showed that an emulsion of these organs can cause 

 the death of a second frog, and an emulsion of its organs 

 frequently produces a typical miliary tuberculosis when 

 injected into a third animal. If these bacilli be now 

 cultivated they show all the characteristics of the bacillus 

 of piscine tubercle, being pathogenic for cold-blooded 

 animals, but no longer for warm-blooded animals, and 

 grow readily at room temperature. It has, however, 

 not been possible up to the present to restore the human 

 or bovine characteristics to these organisms, for failure 

 has resulted from all attempts to successfully immunize 

 a rabbit against them. 



Bertarelli has produced tuberculosis in Varanus by 

 sputum injection. 



Hormann and Morgenroth, Nicolas and Lesieur fed 

 fish (fresh-water) with human sputum containing tubercle 



