SEA-FISHERIES LABORATORY. 223 



Bottle A. — The contents of this bottle were covered 

 with 20 % antiformin solution, which consists of equal 

 parts of soda chlorinata and 15 % liquor sodae, and 

 possesses the property of destroying all other organisms 

 but those which are acid-fast. After remaining in this 

 solution for one hour the deposit was thoroughly washed 

 with sterile saline to remove the antiformin, and then 

 inoculated into twelve tubes of Dorset's egg medium, and 

 incubated at room temperature. 



Bottle B. — The contents of this bottle were treated 

 as in Bottle A, but after thorough washing were injected 

 into two guinea-pigs subcutaneously. 



Bottle C. — The contents of this bottle were emulsified 

 in sterile saline and injected into two guinea-pigs 

 subcutaneously. Some large pieces of tissue which could 

 not be broken up into a satisfactory emulsion were 

 inserted under the skin of the abdomen of two guinea- 

 pigs. 



Since the bacillus of tuberculosis found in cold- 

 blooded animals rarely possess any pathogenicity for the 

 ordinary laboratory animals, there was no expectation 

 that generalised tuberculosis would result in the guinea- 

 pigs. These animals were inoculated in the hope that 

 they would serve as an incubator, and were examined 

 every few days for glandular enlargements and for any 

 lesion at the site of inoculation. 



Only the two animals inoculated with large portions 

 of tissue, untreated with antiformin (Bottle C), showed 

 any reaction. A week after inoculation both animals 

 presented a swelling at the point of injection about the 

 size of a broad-bean. The examination of the two 

 guinea-pigs was conducted on different lines. 



G. P. 37. Thirteen days after inoculation the 

 swelling burst, discharging a creamy caseous pus, and 



