224 TRANSACTIONS LIVERPOOL BIOLOGICAL SOCIETY. 



showed a tendency to form an ulcer. The animal was 

 in good health. It was killed, and a thorough post 

 mortem made after smears and cultures had been made 

 from the pus. All the internal organs were healthy. 



The pus was found to consist almost entirely of 

 polymorphonuclear leucocytes. Many of these were filled 

 with acid-fast bacilli congregated in clumps or bundles 

 consisting of from twenty to forty separate individuals. 



The microphotograph shows the arrangement of 

 these clumps, and also a few organisms lying outside the 

 leucocytes. 



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The second animal inoculated from Bottle C 

 (G. P. 38) with actual masses of the fish-material showed 

 no tendency to ulceration, and was kept under observation 

 for five weeks. The swelling at the site of inoculation 

 showed at no time any tendency to burst, and soon 

 diminished in size until on the 38th day after inocula- 

 tion, when the animal was killed and examined, no trace 

 of the injection except a small nodule of scar tissue was 

 to be found. This scar tissue on section presented the 



