SEA-FISHERIES LABORATORY. 2'25 



appearance of chronic inflammatory fibrous tissue, and 

 no acid-fast bacilli were present. No internal lesions were 

 found. 



The other four guinea-pigs (G. P. 34, 35, 36, 43) 

 inoculated from the emulsified contents of Bottles B 

 and C at no time showed symptoms, were killed on the 

 38th day after inoculation and presented no lesions. 



The cultural results have been unsuccessful in that 

 a pure culture of the organism has not been obtained, 

 and hence it has been impossible to study fully its 

 relationship to human tubercle and the other acid-fast 

 bacilli. The organism has been kept alive, but only in 

 a symbiosis with a Gram-positive coccus. When dilutions 

 are attempted to produce a pure culture the organism dies 

 out at once. 



Conclusions. 



It has been shown that an acid-fast bacillus is 

 present in a skin affection of a cod resembling lupus and 

 containing typical tubercles. The organism is non- 

 pathogenic for the guinea-pig, and does not grow at 

 37° C. It grows at room temperature, but so far only in 

 symbiosis with a coccus. 



